Anti-PRDM14 Polyclonal Antibody for WB, IHC-P, ELISA - Q9GZV8

Core Product Specifications and Parameters

Biological Background: PRDM14 Function and Localization

• PRDM14 (PR domain zinc finger protein 14) is a transcription factor that exerts both positive and negative regulatory effects on gene expression.
• It is critical for maintaining embryonic stem cell identity and enabling somatic cell reprogramming to pluripotency, in part through direct upregulation of the pluripotency factor POU5F1/OCT4 via its proximal enhancer.
• PRDM14 is essential for germ cell development, mediating the reacquisition of pluripotency and epigenetic reprogramming events such as EHMT1 repression.
• The protein localizes predominantly to the nucleus.
• PRDM14 contains an N-terminal PR domain with potential methyltransferase activity and multiple C-terminal zinc finger motifs that facilitate binding to the DNA consensus sequence 5'-GGTC[TC]CTAA-3'.
• Post-translational modifications include phosphorylation, as indicated by proteomic studies.
• Tissue expression is observed in embryonic stem cells, and overexpression has been reported in breast cancer at the protein level.
• Additional functional annotations from UniProt include DNA-binding, metal-binding, methyltransferase, phosphoprotein, transcription, and transcription regulation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 1–260, covering the PR domain; the antibody may recognize full-length PRDM14 as well as N-terminal fragments.
• In Western blotting, expect a predominant band near 64 kDa, consistent with the calculated molecular weight; consider using appropriate molecular weight markers.
• For immunohistochemistry (IHC-P), PRDM14 signals should be nuclear; validate staining specificity in tissues known to express the target, such as embryonic stem cell-derived samples or breast cancer specimens.
• Cross-reactivity with mouse and rat is predicted; always confirm reactivity in your specific experimental system before large-scale studies.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a complete sourcing solution for IVD raw materials, supporting diagnostic manufacturers and researchers from early-stage development to commercialization. For pluripotency and stem cell research applications, we offer a range of validated antibodies, recombinant proteins, and ancillary reagents, including polyclonal antibodies to transcription factors like PRDM14. Our integrated supply network can also accommodate custom sourcing for rare targets and bulk reagent needs, helping to streamline your IVD workflow.