Anti-PRDM12 Polyclonal Antibody for WB, ELISA - Q9H4Q4

Core Product Specifications and Parameters

Biological Background: PRDM12 Function and Localization

• PRDM12 (PR domain zinc finger protein 12) is a transcriptional regulator belonging to the PR/SET domain family, essential for nociceptive neuron development.

• It determines the nociceptive lineage from neural crest cell progenitors, initiating neurogenesis by activating pro-neuronal factors such as NEUROD1, BRN3A, and ISL1, while repressing non-nociceptor fates.

• In adults, PRDM12 maintains nociceptor function by regulating excitability and gene expression in response to pain stimuli, though its expression is restricted to dorsal root ganglia.

• The protein localizes to the nucleus, consistent with its role as a DNA-binding transcription factor, and features multiple zinc-finger domains and a PR domain.

• Mutations in PRDM12 are associated with hereditary sensory and autonomic neuropathy type VIII (HSAN8), linking it to neurodegeneration and pain insensitivity.

• Additional keywords from UniProt include: activator, repressor, triplet repeat expansion, and metal-binding, reflecting its complex regulatory roles.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 100–330 of human PRDM12; thus, the antibody may detect full-length and potentially truncated isoforms. Validate specificity via peptide competition or knockout models.
• For WB, expect a band near 40 kDa (predicted molecular weight). Use lysates from dorsal root ganglia or neuronal cell lines as positive controls, and confirm with molecular weight markers.
• Cross-reactivity with mouse implies epitope conservation; however, verify sequence homology and test on mouse tissue samples (e.g., embryonic or adult dorsal root ganglia) to confirm.
• ELISA can be employed for quantitative analysis. Optimize coating antigen concentration and antibody dilution using purified recombinant PRDM12 or cell lysates.
• Given the restricted expression of PRDM12, enrich specimens from neural crest-derived tissues or employ overexpression systems if endogenous levels are low.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic manufacturers and researchers by providing a comprehensive portfolio of IVD raw materials, including validated antibody pairs, optimized polyclonal and monoclonal antibodies, bulk ancillary reagents, and specialized rare-target sourcing. This PRDM12 antibody supports pain research and nociceptive pathway studies, and we can assist with customized solutions for assay development and scale-up. Partner with CamelBio for end-to-end raw-material supply, from concept to clinic.