Anti-PRCC Polyclonal Antibody for WB and ELISA - Q92733

Core Product Specifications and Parameters

Biological Background: PRCC Function and Localization

• PRCC (Proline-rich protein PRCC) is a proto-oncogene that, when fused with TFE3 via chromosomal translocation, is associated with papillary renal cell carcinoma.
• The protein may regulate cell cycle progression through interaction with MAD2L2 (mitotic arrest deficient 2-like 2), contributing to proper mitotic checkpoint control.
• PRCC is ubiquitously expressed in both fetal and adult tissues, suggesting a fundamental cellular role.
• It localizes predominantly to the nucleus, consistent with its involvement in cell cycle regulation and gene transcription-associated processes.
• Post-translational modifications include phosphorylation, as indicated by proteomics identification; thus it is classified as a phosphoprotein.
• The protein serves as a reference proteome entry (UniProt Q92733) and is a target for proteomics-based discovery.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide covering amino acids 240-491 of human PRCC. This mid-to-C-terminal region likely provides good epitope accessibility; antibody performance should be verified in the relevant sample type.
• Recommended applications include Western blot (predicted molecular weight ~52 kDa) and ELISA. For WB, consider reducing conditions and a whole-cell lysate from human cell lines expressing PRCC.
• Since PRCC is ubiquitously expressed, positive signals can be expected across many human tissue lysates; however, loading controls should be used to confirm specificity.
• For ELISA, the antibody may be used either as a capture or detection reagent after appropriate titration; peptide competition assays can help confirm binding specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, technical services, and consulting, supporting your development from concept to clinic. This anti-PRCC antibody targets a proto-oncogene involved in papillary renal cell carcinoma, fitting into oncology research and diagnostic assay development. Through our extensive network, we can also supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and assist with sourcing rare target raw materials to accelerate your project timelines.