Anti-PRC1 Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - O43663

Core Product Specifications and Parameters

Biological Background: PRC1 Function and Localization

• PRC1 (Protein Regulator of Cytokinesis 1, alias ASE1) is a key mitotic regulator with a predicted molecular mass of 71.6 kDa, encoded by the PRC1 gene (GeneID: 9055). Alternative splicing generates multiple isoforms.
• It functions as a microtubule cross-linking protein, bundling antiparallel microtubules at a spacing of ~35 nm, which is essential for central spindle midzone assembly and successful cytokinesis.
• PRC1 recruits PLK1 to the spindle and is required for KIF14 localization to the central spindle and midbody, thereby promoting downstream signaling (e.g., PLK1-mediated RACGAP1 phosphorylation and ECT2 recruitment) critical for furrow ingression.
• Overexpression of PRC1 has been implicated as an oncogenic driver in bladder cancer, enhancing proliferation, suppressing apoptosis, and facilitating carcinogenesis. Related references: PMID:17409436
• Subcellularly, PRC1 dynamically localizes to the nucleus during interphase, then to the mitotic spindle, spindle midzone, and ultimately the midbody during cytokinesis, also associating with chromosomes.
• Post-translationally, PRC1 is a phosphoprotein and contains coiled-coil domains, consistent with its role in cell cycle-dependent microtubule organization.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal domain (aa 351–620) of human PRC1. This design may recognize multiple isoforms and is predicted to detect endogenous PRC1 in relevant sample systems.
• For Western blotting, a band of approximately 72 kDa is expected. Consider using whole-cell lysates from proliferating cells or synchronized mitotic populations to enhance signal.
• This antibody is validated for IHC-P and IF/ICC. Appropriate fixation and antigen retrieval conditions should be optimized, especially for microtubule-associated targets.
• The oncogenic role of PRC1 in bladder cancer makes it a candidate marker for oncology research; consider validating expression in bladder cancer cell lines or tissue arrays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with streamlined access to high-quality IVD raw materials like this anti-PRC1 polyclonal antibody, along with antibody pairs, bulk ancillary reagents, and support for rare target sourcing. Whether you need validated antibodies for cytokinesis research or customized raw-material solutions for assay development, CamelBio serves as your integrated procurement partner from concept to clinic.