Anti-PRB4 Rabbit Polyclonal Antibody for WB, ELISA - P10163

Core Product Specifications and Parameters

Biological Background: PRB4 Function and Localization

• PRB4 (Basic salivary proline-rich protein 4), also known as Parotid o protein or salivary proline-rich protein II-1, is encoded by the PRB4 gene in humans (Gene ID: 5545).
• It is a secreted protein that contains an N-terminal signal peptide, directing it to the extracellular space upon synthesis.
• The protein belongs to the salivary proline-rich protein family, characterized by high proline content and conserved repeat regions.
• Post-translational modifications include N-terminal pyroglutamic acid formation and glycosylation, as annotated in UniProt (P10163).
• Its calculated molecular weight is approximately 31.3 kDa, closely matching the observed protein weight of 31 kDa.
• While its precise biological function is not fully characterized, salivary proline-rich proteins are generally implicated in oral cavity homeostasis and may interact with calcium or hydroxyapatite.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 178–247 of human PRB4; this region shows homology across mouse and rat, explaining the observed cross-reactivity.
• For Western blotting, a band near 31 kDa is expected; consider using salivary gland lysates or recombinant protein as positive controls.
• In ELISA applications, validate the antibody with known concentrations of PRB4, using saliva or cell culture supernatants as sample sources.
• As a secreted protein, PRB4 may be susceptible to degradation; include protease inhibitors during sample collection and handling.
• Because this is a polyclonal antibody, lot-to-lot performance may vary—recommend pilot testing each batch under standardized conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a trusted partner for IVD raw materials, offering diagnostic manufacturers, laboratories, and research institutions comprehensive access to antibodies, antigens, and ancillary reagents from concept to clinic. For studies involving salivary proteins like PRB4, we provide validated rabbit polyclonal antibodies, monoclonal antibody pairs, and bulk biochemicals to support assay development and biomarker research. Our integrated supply chain also specializes in rare-target sourcing, ensuring you have the critical raw materials needed for your diagnostic workflows.