Anti-PRB4 Polyclonal Antibody for WB, ELISA - P10163

Core Product Specifications and Parameters

Biological Background: PRB4 Function and Localization

• PRB4 is a basic salivary proline-rich protein 4, encoded by the PRB4 gene, and also referred to as parotid o protein or salivary proline-rich protein II-1.
• The protein is secreted into the saliva and contains an N-terminal signal peptide.
• It features repeated sequence elements and is classified as a "Repeat" protein.
• Post-translational modifications include N-terminal pyroglutamic acid formation (pyrrolidone carboxylic acid) and glycosylation, making it a glycoprotein.
• PRB4 has been directly sequenced and is part of the UniProt reference proteome; it has been identified in multiple proteomics studies.
• The precursor protein has a calculated molecular weight of 31,326 Da, approximately 31 kDa.
• No specific disease linkage or detailed functional annotation is provided in current records, though its presence in saliva suggests a role in oral biology.

Experimental Guidance and Technical Tips

• For Western blotting, the antibody is raised against amino acids 178-247 of human PRB4. The predicted band size is ~31 kDa, but glycosylation or other modifications may alter migration. Use a positive control such as human saliva extract or recombinant PRB4.
• In ELISA applications, titrate antibody concentrations to determine optimal signal-to-noise ratio. Consider cross-reactivity with mouse samples as indicated; validate in the relevant sample matrix.
• Since PRB4 is a secreted protein, sample preparation may require collection in protease inhibitors if analyzing bodily fluids. Non-specific binding can be reduced by optimizing blocking conditions.
• As a polyclonal antibody, batch-to-batch variability may occur. For quantitative assays, use consistent reagent lots and standard curves.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a comprehensive one-stop sourcing partner for IVD raw materials, supporting diagnostic developers and researchers with reliable reagents like this anti-PRB4 antibody for oral biology and salivary protein research. Our portfolio includes validated antibody pairs, optimized polyclonal and monoclonal antibodies, and bulk ancillary reagents to streamline your assay development. With expertise in rare target sourcing, we ensure you have consistent access to the raw materials needed from concept to clinical application.