Anti-PRAME Polyclonal Antibody for WB and ELISA - P78395

Core Product Specifications and Parameters

Biological Background: PRAME Function and Localization

• PRAME (Preferentially expressed antigen of melanoma), also known as Opa-interacting protein 4 (OIP-4), is a leucine-rich repeat protein implicated in cancer-testis antigen biology.
• Serves as a substrate-recognition component of a Cul2-RING (CRL2) E3 ubiquitin-protein ligase complex, directing the ubiquitination and proteasomal degradation of specific target proteins.
  Related references: PMID:21822215, PMID:26138980
• The CRL2(PRAME) complex mediates the degradation of truncated MSRB1/SEPX1 selenoproteins that arise from failed UGA/Sec decoding.
  Related references: PMID:26138980
• In the nucleus, CRL2(PRAME) is recruited to epigenetically active promoters bound by the NFY transcription factor, where it contributes to chromatin regulation.
  Related references: PMID:21822215
• Functions as a transcriptional repressor of retinoic acid signaling by inhibiting RARA, RARB, and RARG receptors, thereby blocking retinoic acid-induced cell differentiation and apoptosis.
  Related references: PMID:16179254
• Localizes to multiple cellular compartments, including the nucleus, chromosomes, cytoplasm, Golgi apparatus, and cell membrane.
• Exhibits tissue-specific expression, being primarily detected in the testis, with lower expression in kidney, brain, and skin.
• Post-translational modifications include phosphorylation (Phosphoprotein) and involvement in ubiquitin conjugation (Ubl conjugation pathway), suggesting dynamic regulation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1–200) of human PRAME; therefore, the antibody may recognize epitopes in this domain. For Western blot, consider using PRAME-expressing testis lysates or melanoma cell lines as positive controls.
• With cross-reactivity to human, mouse, and rat, this antibody is suitable for validation across species. Verify reactivity in your model system of interest.
• The predicted molecular weight is approximately 58 kDa; ensure appropriate gel percentage and transfer conditions for optimal detection.
• For ELISA, titrate the antibody against recombinant PRAME protein to determine optimal working dilutions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with comprehensive access to IVD raw materials, technical services, and regulatory consulting. For researchers studying apoptosis, ubiquitination, or melanoma biology, CamelBio offers validated antibody pairs, optimized polyclonal and monoclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to streamline your assay development from concept to clinic.