Anti-PRAF2 Rabbit Polyclonal Antibody for WB, ELISA - O60831

Core Product Specifications and Parameters

Biological Background: PRAF2 Function and Localization

• PRAF2 (JM4/Yip6a) is a member of the PRA1 family proteins involved in intracellular trafficking.
• It may play a role in ER-to-Golgi transport and general vesicular traffic.
• PRAF2 exhibits a proapoptotic function in cerulenin-induced neuroblastoma apoptosis.
• The protein localizes to endosome membranes.
• It is strongly expressed in the brain, particularly in Purkinje cells and cerebellar layers, as well as in small intestine, lung, spleen, and pancreas.
• High expression is observed in neuroblastic tumors and other tumor tissues like breast, colon, lung, and ovary, compared to weaker expression in matched normal tissues.
• It is detected in neuronal cells but not in non-neuronal cells of the cerebral cortex, hippocampus, and lateral ventricles.
• Contains transmembrane helices and is involved in protein transport.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide from the N-terminus (aa 1-50), which may allow detection of full-length PRAF2 and N-terminal variants.
• For Western blot, the predicted molecular weight is ~19 kDa; run a low molecular weight range gel and consider using a positive control from brain or tumor cell lines.
• In ELISA applications, peptide coating or recombinant protein may be used; validate specificity using blocking with the immunogen peptide.
• Cross-reactivity with mouse and rat has been indicated; however, sequence alignment of the immunogen region across species is recommended to confirm expected reactivity.
• Given the role in apoptosis, consider functional studies under cerulenin treatment to assess antibody performance in apoptosis models.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers this PRAF2 rabbit polyclonal antibody as part of our comprehensive IVD raw-material portfolio, supporting research in apoptosis and intracellular trafficking. Our one-stop platform provides validated antibody pairs, bulk reagents, and rare target sourcing to streamline your diagnostic development from concept to clinic.