Anti-PQBP1 Rabbit Monoclonal Antibody for WB, ELISA - O60828

Core Product Specifications and Parameters

Biological Background: PQBP1 Function and Localization

• Intrinsically disordered scaffold protein involved in pre-mRNA splicing, transcription regulation, innate immunity, and neuron development.
  Related references: PMID:10198427 PMID:10332029 PMID:12062018

• Interacts with splicing-related factors via its intrinsically disordered region and regulates alternative splicing of target pre-mRNA species.
  Related references: PMID:10332029 PMID:12062018 PMID:20410308

• Can activate transcription directly or via association with transcription machinery; may suppress POU3F2-mediated transactivation of the DRD1 gene.
  Related references: PMID:10198427

• Involved in ATXN1 mutant-induced cell death and reduces levels of phosphorylated RNA polymerase II large subunit upon interaction with mutant ATXN1.
  Related references: PMID:12062018

• Acts as an innate immune sensor of retroviruses such as HIV by binding reverse-transcribed DNA in the cytosol and activating the cGAS-STING pathway to trigger interferon production.
  Related references: PMID:26046437

• Participates in neuron development and the assembly of cytoplasmic stress granules, possibly via transport of neuronal RNA granules.
  Related references: PMID:21933836

• Localizes predominantly to the nucleus and nuclear speckles, but can also be found in cytoplasmic granules.

• Widely expressed, with highest levels in heart, skeletal muscle, pancreas, spleen, thymus, prostate, ovary, small intestine, and peripheral blood leukocytes.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1–265) of human PQBP1, which includes the WW domain and part of the intrinsically disordered region; this antibody may recognize multiple isoforms produced by alternative splicing.
• For Western blot, the predicted molecular weight is ~30 kDa; confirm band identity using appropriate positive and negative controls.
• Cross-reactivity with mouse and rat has been documented; validate the antibody in your specific model system for consistent results.
• Consider using this antibody to study protein–protein interactions via co-immunoprecipitation, as PQBP1 associates with splicing factors, transcription regulators, and viral DNA.
• For ELISA applications, optimize coating conditions and antibody dilution; non-specific binding may occur due to the target's disordered nature.
• Store and handle according to recommended conditions (shipped on ice bag); avoid repeated freeze-thaw cycles to maintain stability.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop partner for IVD raw materials, providing high-quality antibodies like this PQBP1 monoclonal antibody to support research in neuroscience, immunology, and oncology. We offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target sourcing to streamline your procurement process. Trust CamelBio to deliver reliable, scalable supplies from concept to clinic.