Anti-PPP6R2 Rabbit Polyclonal Antibody for WB and ELISA - O75170

Core Product Specifications and Parameters

Biological Background: PPP6R2 Function and Localization

• Encoded by the PPP6R2 gene (also designated PP6R2, SAPS2, SAP190, KIAA0685), the protein is a regulatory subunit of serine/threonine-protein phosphatase 6 (PP6). This 105 kDa polypeptide belongs to the SAPS domain family, which typifies PP6 regulatory subunits.
• Functions as a scaffolding subunit that directs PP6-mediated dephosphorylation of NFKBIE (IκB-ε), thereby counteracting its ubiquitin-dependent degradation upon TNF-α stimulation. This mechanism modulates NF-κB signaling.
• Predominantly localizes to the cytoplasm, consistent with its role in cytoplasmic signal transduction.
• Ubiquitously expressed across tissues, with the highest levels detected in testis, followed by liver, heart, kidney, brain, and placenta, suggesting broad physiological significance.
• Identified as a phosphoprotein in large-scale proteomic studies, post-translational modifications may regulate its activity or interactions.
• Alternative splicing of the PPP6R2 transcript gives rise to multiple protein isoforms, expanding the functional repertoire in different cellular contexts.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 640-959 of human PPP6R2 (isoform corresponding to NP_001229827.1), which lies in the C-terminal region. This design may enable recognition of multiple isoforms, but it is advisable to verify specificity using positive controls such as human cell lines with confirmed PPP6R2 expression.
• For Western blotting, a band of approximately 105 kDa is expected under reducing conditions. Use lysates from tissues with high PPP6R2 abundance (e.g., testis, liver) for initial optimization. Include appropriate loading controls and consider a blocking agent that minimizes background.
• In ELISA applications, direct coating of the recombinant protein or peptide may be used for detection, but antibody titration and competition with the immunogen are recommended to assess binding specificity.
• As a polyclonal antibody, lot-to-lot variability can occur. Establish a bridging study when switching lots to ensure consistent performance across experiments. Additionally, confirm cross-reactivity with your target species if deviating from the verified human specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers IVD developers with comprehensive access to raw materials and technical support, from early R&D to clinical application. This PPP6R2 polyclonal antibody is part of our extensive catalog of immune reagents tailored for cell signaling and oncology research. Beyond single antibodies, we supply matched antibody pairs, optimized monoclonal options, and bulk ancillary reagents to accelerate your assay development and manufacturing workflows.