Anti-PPP6C Rabbit Monoclonal Antibody for WB, ELISA - O00743

Core Product Specifications and Parameters

Biological Background: PPP6C Function and Localization

• PPP6C (also known as PP6 or PP6C) is the catalytic subunit of serine/threonine-protein phosphatase 6 (PP6), a key enzyme in the phosphoprotein phosphatase family. Related references: PMID:17079228 PMID:29053956 PMID:32474700

• PP6 regulates cell cycle progression through IL2 receptor signaling and restricts G1 to S phase transition in cancer cells via control of cyclin D1. Related references: PMID:10227379 PMID:17568194

• During mitosis, PP6 is essential for proper spindle positioning. Related references: PMID:27335426

• PP6 down-regulates MAP3K7 kinase activation in the IL1 signaling pathway by dephosphorylating MAP3K7. Related references: PMID:17079228

• In innate immune defense against viruses, PP6 dephosphorylates RIGI, an essential step that triggers RIGI-mediated signaling activation. Related references: PMID:29053956

• PP6 acts as a negative regulator of the cGAS-STING pathway by mediating dephosphorylation and inactivation of CGAS and STING1; CGAS dephosphorylation at Ser-435 impairs GTP binding. Related references: PMID:32474700 PMID:32753499

• Subcellularly, PPP6C localizes to the cytoplasm and mitochondria.

• Ubiquitously expressed, with highest levels in testis, heart, kidney, brain, stomach, liver, and skeletal muscle.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a conserved internal region (aa 206–305) of human PPP6C; verify sequence alignment with mouse and rat to confirm expected cross-reactivity.
• For Western blot, a band at approximately 35 kDa is expected; optimize lysis and reducing conditions to obtain a clear signal.
• In ELISA applications, titrate the coating antigen and antibody to achieve the desired sensitivity and specificity.
• Given PPP6C's roles in cell cycle and innate immunity, consider analyzing expression in synchronized cell populations or immune-stimulated samples.

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CamelBio is your dedicated one-stop sourcing partner for IVD raw materials, supporting diagnostic manufacturers from concept to clinic. For cell cycle and innate immunity research, we offer validated antibody pairs, optimized monoclonal antibodies like this anti-PPP6C clone, and bulk ancillary reagents. Our global supply network also efficiently sources rare and emerging target raw materials for specialized immunoassay development.