Anti-PPP5C Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P53041

Core Product Specifications and Parameters

Biological Background: PPP5C Function and Localization

• Serine/threonine-protein phosphatase that dephosphorylates a variety of signaling proteins such as CSNK1E, ASK1/MAP3K5, PRKDC, RAF1, nuclear receptors (NR3C1, PPARG, ESR1, ESR2), SMAD proteins, and TAU/MAPT, regulating multiple cellular processes. Related references: PMID:14734805, PMID:14764652, PMID:14871926

• Plays a crucial role in DNA damage response by participating in ATM/ATR-signaling, dephosphorylating PRKDC and TP53BP1, thereby influencing checkpoint activation and DNA repair. Related references: PMID:14871926, PMID:16260606, PMID:21144835

• Inhibits ASK1/MAP3K5-mediated apoptosis induced by oxidative stress, promoting cell survival. Related references: PMID:23102700

• Regulates circadian rhythms via dephosphorylation and activation of CSNK1E. Related references: PMID:16790549

• Modulates TGF-beta signaling through regulation of SMAD3 phosphorylation and protein expression. Related references: PMID:22781750

• Dephosphorylates TAU/MAPT, potentially contributing to neurodegenerative disease mechanisms. Related references: PMID:15546861

• Dephosphorylates FNIP1, disrupting interaction with HSP90AA1/Hsp90. Related references: PMID:30699359

• Ubiquitously expressed and localized to the nucleus, cytoplasm, and cell membrane.

Experimental Guidance and Technical Tips

• The immunogen is designed against the N-terminal region (aa 1-210), encompassing the TPR domain; this region facilitates interactions with HSP90 and other proteins, potentially ensuring recognition of native PPP5C.
• For Western blotting, the predicted molecular weight is 57 kDa; use appropriate gel percentage for this size range and validate in the relevant sample system.
• Cross-reactivity with mouse and rat suggests utility in rodent models; confirm by comparing with known positive controls.
• For immunofluorescence (IF/ICC), consider fixation and permeabilization conditions to allow detection of PPP5C in the nucleus, cytoplasm, and membrane compartments.
• In ELISA, the polyclonal nature may provide high sensitivity; perform titration experiments to determine optimal working concentration.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a comprehensive provider of IVD raw materials, offering validated antibody pairs, monoclonal and polyclonal antibodies, and ancillary reagents for diagnostic manufacturing. This anti-PPP5C antibody, targeting a key phosphatase involved in cell signaling and DNA damage response, supports research and assay development. Our extensive supply chain ensures reliable bulk sourcing for various research and diagnostic applications.