Anti-PPP4R3B Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q5MIZ7

Core Product Specifications and Parameters

Biological Background: PPP4R3B Function and Localization

• PPP4R3B, also known as SMEK2, is a regulatory subunit of serine/threonine-protein phosphatase 4 (PP4), which modulates PP4 catalytic activity and substrate specificity.
• It is encoded by the PPP4R3B gene in humans and undergoes alternative splicing, producing multiple isoforms.
• The protein may regulate PPP4C activity at centrosomal microtubule organizing centers, implicating it in centrosome function and microtubule organization.
• Subcellularly, PPP4R3B localizes to the cytoplasm, centrosomes (microtubule organizing centers), and the nucleus, consistent with its role in PP4 signaling.
• The protein is moderately expressed across various tissues and specific brain regions, suggesting a broad but regulated functional role.
• PPP4R3B is classified as a phosphoprotein, indicating that its activity or interactions may be modulated by phosphorylation.
• As a reference proteome entry with proteomics identification, its existence and expression have been experimentally confirmed at the protein level.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 628-817, targeting the C-terminal region of PPP4R3B; this design may enhance specificity for this regulatory subunit.
• For Western blot (WB), consider using standard polyclonal antibody dilutions (e.g., 1:500-1:2000) and validate in lysates from HeLa, HEK293, or mouse brain tissues, given the reported moderate expression.
• In immunofluorescence/immunocytochemistry (IF/ICC), centrosomal localization may be observed; co-staining with gamma-tubulin or pericentrin is recommended to confirm targeting to the microtubule organizing center.
• Cross-reactivity with mouse and rat is reported; validate in the relevant species’ sample systems to ensure reactivity and specificity.
• For ELISA, the antibody’s polyclonal nature may provide robust detection, but appropriate controls and titration are essential for quantitative assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD manufacturers and researchers, offering validated antibody pairs, bulk ancillary reagents, and rare target raw materials. For PPP4R3B research, we can assist with customized polyclonal antibody production and optimization to support your diagnostic or assay development needs. Our one-stop approach streamlines procurement from concept to clinic, ensuring reliable supply chain management for your projects.