Anti-PPP4R1 Rabbit Polyclonal Antibody for WB, IP, ELISA - Q8TF05

Core Product Specifications and Parameters

Biological Background: PPP4R1 Function and Localization

• PPP4R1, also known as MEG1 or PP4R1, is a regulatory subunit of serine/threonine-protein phosphatase 4 (PP4). It plays a critical role in substrate specificity and localization of the phosphatase complex.
• The PPP4C-PPP4R1 complex dephosphorylates HDAC3, a key histone deacetylase, implicating it in chromatin remodeling and gene expression regulation.
• PPP4R1 is involved in the negative regulation of TNF-induced NF-kappa-B signaling by controlling the dephosphorylation of TRAF2, thereby attenuating inflammatory responses.
• During microbial infection, PPP4R1 bridges merkel polyomavirus small tumor antigen with NEMO, contributing to viral inhibition of NF-kappa-B.
• PPP4R1 is widely expressed, with particularly high levels in cultured mesangial cells, and isoforms 1 and 2 are specifically expressed in renal tissues, suggesting a role in renal cell division.
• The protein undergoes alternative splicing and is a phosphoprotein, indicating regulatory post-translational modifications.

Experimental Guidance and Technical Tips

• The immunogen spans a region from amino acid 324 to 647, covering a substantial portion of the protein. Consider verifying the expected band size of 107 kDa in WB using appropriate molecular weight markers.
• Optimize primary antibody dilution and incubation conditions for each application (WB, IP, ELISA) using positive control lysates from human or mouse cell lines or tissues, such as renal mesangial cells.
• For IP, include appropriate IgG controls to confirm specific pulldown and evaluate co-immunoprecipitation of PPP4C or other interaction partners.
• Validate cross-reactivity in mouse samples by comparing sequence homology of the immunogen region and testing on mouse tissue lysates.
• Due to alternative splicing, multiple isoforms may be detected; confirm by using isoform-specific controls or siRNA knockdown.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with seamless access to high-quality IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target sourcing for challenging targets like PPP4R1. With expertise in NF-kappa-B signaling, cell cycle regulation, and infectious disease research, we support your assay development from concept to clinic, ensuring reliable supply and technical consultation every step of the way.