Anti-PPP4C Rabbit Polyclonal Antibody for WB, IHC-P, IP, ELISA - P60510

Core Product Specifications and Parameters

Biological Background: PPP4C Function and Localization

• PPP4C encodes the catalytic subunit of serine/threonine-protein phosphatase 4 (PP4), also known as protein phosphatase X (PP-X). It is a member of the PPP family of protein phosphatases.
• PP4 is a multifunctional enzyme involved in critical cellular processes including microtubule organization at centrosomes, maturation of spliceosomal snRNPs, apoptosis, DNA repair, and tumor necrosis factor (TNF)-alpha signaling.
• The PP4 holoenzyme complexes, such as PPP4C-PPP4R1 and PPP4C-PPP4R2-PPP4R3A, regulate histone deacetylation by dephosphorylating HDAC3 and participate in DNA damage response by specifically dephosphorylating gamma-H2AX at Ser-140.
• Subcellular localization includes the cytoplasm, nucleus, and centrosomes, consistent with its roles in cell cycle progression, DNA damage checkpoint signaling, and cytoskeletal organization.
• PPP4C dephosphorylates NDEL1 at CDK1 phosphorylation sites, negatively regulating CDK1 activity during interphase, and dephosphorylates RPA2 to facilitate RAD51 recruitment to chromatin for homologous recombination repair.
• The catalytic activity requires manganese ions as cofactors, and the protein is subject to post-translational modifications including acetylation and methylation.
• Additional functions include activation of c-Jun N-terminal kinase MAPK8, regulation of NF-kappa-B activation, and modulation of cell migration, implicating PP4 in immune and metastatic pathways.

Experimental Guidance and Technical Tips

• The immunogen corresponds to full-length human PPP4C (amino acids 1-307), making this polyclonal antibody suitable for detecting endogenous PP4 across multiple applications.
• For Western blotting (WB), a band at approximately 35 kDa is expected. Consider using phosphatase inhibitors and positive control lysates from HeLa or HEK293 cells.
• In immunohistochemistry-paraffin (IHC-P), optimize antigen retrieval and antibody dilution based on tissue-specific expression patterns. Nuclear and cytoplasmic staining may be observed.
• For immunoprecipitation (IP), the antibody can capture native PPP4C complexes; titrate antibody concentration and ensure adequate washing to reduce background.
• Cross-reactivity with mouse and rat orthologs has been validated, enabling studies in common model organisms.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with streamlined access to high-quality IVD raw materials. This anti-PPP4C antibody supports studies in DNA damage and repair, centrosome biology, and phosphatase signaling cascades. Beyond primary antibodies, our collaborative network offers validated antibody pairs, custom monoclonal development, and bulk ancillary reagents to accelerate your assay development from concept to clinic. For specialized or rare targets in the phosphatase pathway, our sourcing experts can help identify and supply optimal raw materials tailored to your IVD needs.