Anti-PPP4C Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, IP, ELISA - P60510

Core Product Specifications and Parameters

Biological Background: PPP4C Function and Localization

• PPP4C encodes the catalytic subunit of serine/threonine-protein phosphatase 4 (PP4), also known as protein phosphatase X (PP-X).
• PP4 is a ubiquitous phosphatase involved in numerous cellular processes, including microtubule organization at centrosomes, spliceosomal snRNP maturation, apoptosis, DNA repair, TNF-alpha signaling, JNK activation, histone acetylation regulation, DNA damage checkpoint signaling, NF-kappa-B activation, and cell migration.
• PP4 forms distinct complexes with regulatory subunits such as PPP4R1, PPP4R2, and PPP4R3A, which confer substrate specificity: for example, the PPP4C-PPP4R2-PPP4R3A complex dephosphorylates gamma-H2AX at DNA damage sites, while the PPP4C-PPP4R1 complex may regulate HDAC3.
• The protein localizes predominantly to the cytoplasm and nucleus, with enrichment at the centrosome and microtubule organizing center, consistent with its roles in cytoskeletal regulation and mitosis.
• Post-translational modifications include acetylation and methylation, and the enzyme requires manganese as a cofactor for its hydrolase activity.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant fusion protein covering amino acids 1-307 of human PPP4C (NP_002711.1), which represents the full-length catalytic subunit; this design is expected to generate polyclonal antibodies recognizing multiple epitopes.
• For Western blot analysis, a major band near 35 kDa is anticipated; however, post-translational modifications or complex formation may result in additional bands—validate in the relevant sample system.
• In immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), consider that PPP4C localizes to both cytoplasm and nucleus, with centrosomal enrichment; appropriate fixation and permeabilization protocols should be tested.
• For immunoprecipitation (IP) and ELISA applications, standard conditions can be used; note that as a polyclonal preparation, cross-linking to beads or using a bridging antibody may improve capture efficiency.
• Cross-reactivity with mouse and rat orthologs is supported, but sequence alignment and preliminary confirmation in the intended species are recommended before high-throughput assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a one-stop sourcing bridge for IVD raw materials, connecting diagnostic manufacturers, labs, and research institutes with validated antibody pairs, optimized polyclonal and monoclonal antibodies, bulk ancillary reagents, and rare-target raw-material procurement. The PPP4C antibody supports research into DNA repair, cell cycle control, and stress signaling pathways, with CamelBio facilitating access to complementary reagents and custom services for assay development and scale-up.