Anti-PPP3R2 Polyclonal Antibody for WB, ELISA - Q96LZ3

Core Product Specifications and Parameters

Biological Background: PPP3R2 Function and Localization

• PPP3R2, also known as Calcineurin subunit B type 2 or Calcineurin B-like protein (CBLP), is a regulatory subunit of calcineurin, a calcium/calmodulin-dependent serine/threonine protein phosphatase.
• As a regulatory subunit, PPP3R2 binds calcium ions and confers calcium sensitivity to the calcineurin catalytic subunit, thereby regulating phosphatase activity in response to intracellular calcium levels.
• PPP3R2 belongs to the calcineurin B subunit family and shares homology with PPP3R1, but it is a testis-specific isoform encoded by the PPP3R2 gene (GeneID: 5535).
• Subcellular localization studies indicate that PPP3R2 is primarily localized to the mitochondrion, suggesting a role in mitochondrial calcium signaling pathways.
• The protein contains myristoylation and metal-binding sites, which are important for membrane association and calcium coordination, respectively.
• Expression of PPP3R2 is restricted to testis tissue, implying specialized functions in sperm development or testicular physiology.

Experimental Guidance and Technical Tips

• The immunogen used spans amino acids 1-173, encompassing nearly the entire protein. This design may favor recognition of the native conformation in applications such as ELISA and denaturing Western blot.
• For Western blot, consider testing a range of primary antibody concentrations and validate with a positive control known to express PPP3R2 (e.g., human testis lysate or transfected cell lines).
• In ELISA, direct or sandwich formats can be explored; using recombinant PPP3R2 protein as a standard and optimizing the coating concentration is recommended.
• Cross-reactivity with mouse and rat suggests utility in rodent models, but experimental validation in the relevant species-specific sample system is advised.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, research laboratories, and institutes with a comprehensive one-stop sourcing platform for IVD raw materials, technical services, and consulting—supporting projects from early-stage concept to clinical application. This anti-PPP3R2 polyclonal antibody targets a testis-specific calcineurin subunit and fits into research areas such as calcium signaling and male fertility. Through our global supply network, we can rapidly deliver validated antibody pairs, bulk purified monoclonal and polyclonal antibodies, ancillary reagents, and custom sourcing for rare target raw materials to accelerate your assay development.