Anti-PPP3R1 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P63098

Core Product Specifications and Parameters

Biological Background: Calcineurin Subunit B Type 1 Function and Localization

• Encoded by the PPP3R1 gene, calcineurin subunit B type 1 (also known as CNB, CNB1, or CALNB1) functions as the regulatory subunit of the calcium/calmodulin-dependent serine/threonine protein phosphatase calcineurin.
• It confers calcium sensitivity to the calcineurin holoenzyme, thereby enabling calcium-dependent signal transduction pathways.
• The protein contains calcium-binding sites and is a member of the calcineurin regulatory subunit family.
• Subcellularly, it localizes to the cytosol and cell membrane, including the sarcolemma, facilitating its role in relaying calcium signals at various cellular compartments.
• Post-translational modifications include N-terminal myristoylation, which likely aids membrane association, and phosphorylation, which may regulate its activity.
• The mature protein is approximately 19 kDa, consistent with its role as a compact regulatory subunit.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 11-170 of human PPP3R1, covering a large portion of the protein; therefore, the antibody may detect multiple epitopes.
• For Western blot, consider the predicted molecular weight of 19 kDa and optimize gel percentage and transfer conditions accordingly.
• In immunofluorescence/immunocytochemistry, account for cytoplasmic and membrane localization when selecting fixation and permeabilization protocols.
• The antibody cross-reacts with mouse and rat species; validate in the relevant sample system to confirm performance.
• For ELISA, optimal antibody dilution should be determined empirically.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic manufacturers, laboratories, and research institutes with a comprehensive one-stop portfolio of IVD raw materials. For projects targeting calcineurin signaling and calcium-dependent pathways, we offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to streamline your assay development from concept to clinic.