Anti-PPP3CC Polyclonal Antibody for WB, ELISA - P48454

Core Product Specifications and Parameters

Biological Background: PPP3CC Function and Localization

• Encoding the gamma isoform of calcineurin A catalytic subunit, PPP3CC is a serine/threonine-protein phosphatase also known as CAM-PRP catalytic subunit, testis-specific catalytic subunit, or calmodulin-dependent calcineurin A subunit gamma.
• Functions as a calcium/calmodulin-dependent phosphatase that transduces intracellular Ca2+ signals, playing an essential role in multiple signaling pathways.
• Dephosphorylates and activates transcription factor NFATC1, thereby promoting its nuclear translocation and downstream gene expression.
• Dephosphorylates and inactivates transcription factor ELK1, providing a cross-talk between calcium signaling and the MAP kinase pathway.
• Dephosphorylates DARPP32, a key regulator of dopaminergic signal transduction, linking calcineurin activity to neurological processes.
• Localizes predominantly to mitochondria, suggesting a specialized function in mitochondrial calcium signaling and metabolic regulation.
• Highly expressed in the testis, indicating a specific role in male germ cell development and function.
• Characterized by alternative splicing, calmodulin-binding, and metal ion cofactors (iron, zinc), with phosphoprotein features underscoring its dynamic regulation.

Experimental Guidance and Technical Tips

• The immunogen (aa 373-512 of human PPP3CC) falls within a region that may contain conserved motifs; consider potential isoform or species cross-reactivity and validate in the relevant sample system.
• For Western blotting, the predicted molecular weight is 58 kDa; compare with endogenous expression profiles in testis-derived cell lines or tissue lysates.
• ELISA applications may benefit from optimizing coating and detection conditions with recombinant PPP3CC protein as a standard.
• Use appropriate calcium/calmodulin activation controls in functional studies to confirm target engagement.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supplies this anti-PPP3CC polyclonal antibody as part of a comprehensive portfolio for calcium signaling and phosphatase research. As a dedicated IVD raw material provider, we offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents to streamline assay development. Our sourcing capability extends to rare target raw materials, ensuring diagnostic manufacturers and research institutes have a reliable bridge from concept to clinic.