Anti-PPP2R3B Polyclonal Antibody for WB and ELISA - Q9Y5P8

Core Product Specifications and Parameters

Biological Background: PPP2R3B Function and Localization

• PPP2R3B (also known as PR48, NYREN8, PPP2R3L) encodes a regulatory B subunit of protein phosphatase 2A (PP2A), a major serine/threonine phosphatase involved in many cellular processes.
• This subunit is thought to modulate substrate selectivity and catalytic activity of the PP2A holoenzyme, and may direct the phosphatase to specific subcellular compartments.
• The protein localizes to the nucleus, suggesting a role in nuclear PP2A signaling events.
• Alternative splicing generates multiple isoforms of PPP2R3B, which may have distinct regulatory functions.
• The protein contains calcium-binding motifs and is a metal-binding protein, indicating potential regulation by divalent cations.
• Structural studies (3D-structure keyword) provide insights into its interaction with other PP2A subunits and substrates.
• Its identification in proteomics screens (Proteomics identification keyword) underscores its broad expression and biological relevance.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 356-575 of human PPP2R3B, a C-terminal region that may be conserved across some splice variants; thus, the antibody could potentially recognize multiple isoforms. Verify specificity using isoform-specific controls if needed.
• For Western blot (WB), a band around 65 kDa is expected; however, actual migration may vary depending on post-translational modifications. Include appropriate molecular weight markers and validate in relevant cell or tissue lysates (e.g., human or mouse nuclear fractions).
• For ELISA, consider titrating the antibody and using a suitable coating buffer; cross-reactivity with mouse PPP2R3B may allow detection in mouse samples. Normalize signals to protein concentration.
• Because PPP2R3B functions in the nucleus, subcellular fractionation or immunofluorescence may be used to confirm its localization, though these applications have not been validated with this antibody.
• Always include negative controls (e.g., knockdown or knockout lysates) to confirm specificity in your experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research laboratories with a comprehensive portfolio of IVD raw materials, including validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. For studies on PP2A signaling and phosphatase regulation, we offer this PPP2R3B antibody alongside a range of related products to streamline your workflow. Our sourcing capabilities also extend to rare target raw materials, ensuring a reliable supply for your projects from concept to clinic.