Anti-PPP2R2B/PPP2R2C Polyclonal Antibody for WB, ELISA - Q00005 / Q9Y2T4

Core Product Specifications and Parameters

Biological Background: PPP2R2B/PPP2R2C Function and Localization

• PPP2R2B (also known as PR55-beta) is a regulatory subunit of serine/threonine-protein phosphatase 2A (PP2A), a major cellular phosphatase involved in numerous signaling pathways.
• It acts as a B regulatory subunit that modulates substrate selectivity and catalytic activity of the PP2A catalytic core, and directs the holoenzyme to specific subcellular compartments.
• Isoform 2 of PPP2R2B promotes proapoptotic activity and regulates neuronal survival by balancing mitochondrial fission and fusion.
• PPP2R2B is predominantly expressed in the brain, highlighting its critical role in neuronal function and its association with neurodegenerative disorders.
• The protein localizes to the cytoplasm, cytoskeleton, membrane, and mitochondria, particularly the mitochondrial outer membrane.
• It belongs to the PPP2R family of regulatory subunits and contains WD repeat domains important for protein-protein interactions.
• Post-translational modifications include phosphorylation, and it is implicated in diseases such as spinocerebellar ataxia.
• This antibody targets a shared sequence in PPP2R2B and PPP2R2C, enabling detection of both isoforms.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 108-220 of human PPP2R2B/PPP2R2C, a region conserved between the two isoforms; consider this when assessing isoform specificity.
• For Western blotting (WB), validate the antibody in relevant cell or tissue lysates, such as brain tissue where PPP2R2B is highly expressed.
• When performing ELISA, use full-length recombinant protein as positive control and include appropriate negative controls to confirm binding specificity.
• Due to the high conservation between PPP2R2 family members, cross-reactivity with other B subunits (e.g., PPP2R2A) may occur; verify by siRNA or knockout models if necessary.
• The antibody is supplied as a polyclonal rabbit antibody; for reproducible results, consider using the same lot for comparisons across experiments.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your dedicated IVD raw-material partner, providing validated antibody pairs, optimized polyclonal and monoclonal antibodies, and ancillary reagents for diagnostic development. This anti-PPP2R2B/PPP2R2C antibody supports research in cell signaling, apoptosis, and neurological disease studies. Beyond this product, CamelBio can assist with sourcing rare targets, custom antibody development, and technical consulting to accelerate your projects from concept to clinic.