Anti-PPP2R1A/PPP2R2A/PPP2R1B Rabbit Polyclonal Antibody for WB, ELISA - P30153 / P63151 / P30154

Core Product Specifications and Parameters

Biological Background: PPP2R1A/PPP2R2A/PPP2R1B Function and Localization

• PPP2R1A (PR65-alpha) is the scaffolding subunit of protein phosphatase 2A (PP2A), coordinating assembly of catalytic and regulatory B subunits.
  Related references: PMID:15525651, PMID:16580887, PMID:33243860
• Essential for proper chromosome segregation and centromeric localization of SGO1 during mitosis.
  Related references: PMID:16580887
• Component of STRIPAK complexes, regulating signaling pathways (Hippo, MAPK, nuclear receptor) and processes like cell growth, differentiation, and apoptosis.
  Related references: PMID:18782753, PMID:33633399
• Functions in the INTAC complex to dephosphorylate RNA polymerase II CTD, mediating transcriptional quality checkpoint and premature termination.
  Related references: PMID:33243860, PMID:34004147
• Mediates AKT1 dephosphorylation at Ser-473 via RACK1, preventing AKT-mTOR signaling; critical for regulatory T-cell homeostasis.
• Regulates SKA complex recruitment to kinetochores.
  Related references: PMID:28982702
• Localizes to cytoplasm, nucleus, chromosome centromeres, lateral cell membrane, and dendrites.
• Post-translational modifications include acetylation; associated with intellectual disability disease variants and host-virus interactions.

Experimental Guidance and Technical Tips

• The immunogen encompasses the N-terminal region (amino acids 1-161) of PPP2R1A, which is highly conserved across human, mouse, and rat, consistent with the reported cross-reactivity.
• For Western blotting, a band around 65 kDa is expected; validate in lysates from cell lines or tissues known to express PPP2R1A.
• In ELISA, the polyclonal nature may provide signal amplification; consider pairing with a monoclonal antibody for sandwich ELISA if required.
• Since PPP2R2A and PPP2R1B share epitopes within the immunogen region, cross-recognition is expected; verify specificity using knockdown or knockout controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive one-stop sourcing platform for IVD raw materials, offering high-quality antibodies like this anti-PPP2R1A/PPP2R2A/PPP2R1B polyclonal antibody to support research into PP2A complex biology and signaling. Beyond individual antibodies, we provide validated antibody pairs, optimized monoclonal/polyclonal preparations, and bulk ancillary reagents to streamline your assay development. For rare or custom targets, our sourcing network facilitates access to specialized raw materials, bridging the gap from concept to clinic.