Anti-PPP1R9B Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q96SB3

Core Product Specifications and Parameters

Biological Background: PPP1R9B Function and Localization

• PPP1R9B encodes Neurabin-2 (also known as spinophilin or protein phosphatase 1 regulatory subunit 9B), an actin-binding scaffold protein involved in multiple signaling pathways.
• It modulates excitatory synaptic transmission and dendritic spine morphology by cross-linking actin filaments (F-actin) and targeting protein phosphatase 1 (PP1) to dendritic spines, regulating its specificity toward ion channels and glutamate receptors (AMPA and NMDA types).
• Spinophilin links the actin cytoskeleton to the plasma membrane at synaptic junctions and plays a role in G protein-coupled receptor signaling, including dopamine D2 and alpha-adrenergic receptors, by forming signaling complexes.
• It is required for hepatocyte growth factor (HGF)-induced cell migration and may regulate p70 S6 kinase activity by forming a complex with TIAM1.
• The protein localizes to multiple subcellular compartments including the cytoplasm, cytoskeleton, nucleus, dendritic spines, postsynaptic density, adherens junctions, lamellipodia, filopodia, and ruffle membranes.
• Keywords indicate involvement in neurogenesis, differentiation, and development, and the protein is classified as a phosphoprotein.

Experimental Guidance and Technical Tips

• For Western blot, consider using whole-cell lysates from human or mouse brain tissues, as PPP1R9B is enriched in neuronal cells. The predicted molecular weight is approximately 89 kDa. Validate antibody specificity in your sample system.
• For immunofluorescence/immunocytochemistry, employ fixation and permeabilization protocols appropriate for cytoskeletal and membrane-associated targets. Include appropriate controls to confirm localization patterns.
• ELISA applications may require optimization of coating antigen and detection conditions; consider using the immunogen peptide as a positive control.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, research labs, and institutes with one-stop access to IVD raw materials, technical services, and consulting, covering every stage from concept to clinic. This anti-PPP1R9B polyclonal antibody is suitable for research into synaptic transmission and actin cytoskeleton dynamics. For validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing, contact CamelBio as your procurement partner.