Anti-PPP1R7 Rabbit Polyclonal Antibody for WB, ELISA - Q15435

Core Product Specifications and Parameters

Biological Background: PPP1R7 Function and Localization

• Protein phosphatase 1 regulatory subunit 7 (PPP1R7), also known as SDS22 or protein phosphatase 1 regulatory subunit 22, is a regulatory subunit of protein phosphatase 1 (PP1).
• PPP1R7 modulates the activity and substrate specificity of PP1, playing a crucial role in various cellular processes including cell cycle progression and glycogen metabolism.
• The protein contains leucine-rich repeats, which are involved in mediating protein-protein interactions and are characteristic of a diverse family of proteins.
• Encoded by the PPP1R7 gene, alternative splicing generates multiple transcript variants, potentially expanding its functional repertoire.
• Subcellularly, PPP1R7 localizes predominantly to the nucleus, consistent with its role in regulating nuclear PP1 targets.
• PPP1R7 is widely expressed across human tissues, indicating a broad functional relevance in different cell types.
• Post-translational modifications, including acetylation and phosphorylation, have been reported, suggesting dynamic regulation of its activity and interactions.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 281-360 of human PPP1R7, a region that may be conserved across species, supporting the observed cross-reactivity with human and mouse.
• For Western blot, a band around 42 kDa is expected; consider using whole-cell or nuclear extracts as the target localizes to the nucleus.
• As a polyclonal antibody, it offers robust detection sensitivity; however, lot-to-lot variability should be assessed, and each new lot validated in the relevant sample system.
• For ELISA applications, optimize coating antigen and antibody dilution conditions to achieve the desired signal-to-noise ratio.
• When working with mouse samples, verify the reactivity in your specific mouse strain and tissue, as expression levels may vary.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with comprehensive access to IVD raw materials, including validated antibody pairs and optimized polyclonal antibodies like this anti-PPP1R7 antibody. Supporting the phosphatase research area, we offer one-stop sourcing for monoclonal antibodies, bulk ancillary reagents, and rare-target raw materials to streamline your assay development from concept to clinic. Partner with CamelBio to secure high-quality reagents and accelerate your diagnostic projects.