Anti-PPP1R3B Rabbit Polyclonal Antibody for WB, ELISA - Q86XI6

Core Product Specifications and Parameters

Biological Background: PPP1R3B Function and Localization

• Protein phosphatase 1 regulatory subunit 3B (PPP1R3B), also known as GL, PTG, or PPP1R4, is a glycogen-targeting subunit for protein phosphatase 1 (PP1).
• PPP1R3B facilitates the interaction of PP1 with enzymes of glycogen metabolism, suppressing PP1-mediated dephosphorylation (inactivation) of glycogen phosphorylase and enhancing glycogen synthase activation, thereby limiting glycogen breakdown and promoting glycogen synthesis.
• Overexpression of PPP1R3B in hepatocytes dramatically increases basal and insulin-stimulated glycogen synthesis (By similarity).
• PPP1R3B is highly expressed in liver and at lower levels in skeletal muscle (vastus lateralis, gastrocnemius, soleus) at the protein level; mRNA is highest in skeletal muscle, moderate in liver and heart, and weak in placenta and lung.
• It belongs to the protein phosphatase 1 regulatory subunit family and is a phosphoprotein.
• Annotations include involvement in carbohydrate and glycogen metabolism, and it has a predicted molecular weight of approximately 33 kDa.

Experimental Guidance and Technical Tips

• The immunogen (aa 1-140) targets the N-terminus of human PPP1R3B; consider that this region may be conserved across species (cross-reactivity with mouse and rat is reported) and may detect potential isoforms.
• For Western blot, the predicted molecular weight is ~33 kDa; validate band specificity using positive control lysates from liver or skeletal muscle tissues, taking into account species cross-reactivity.
• In ELISA applications, optimize coating and detection conditions for quantitative analysis; use recombinant protein or peptide controls to establish assay sensitivity.
• Note that expression levels may be higher in liver and muscle; consider tissue-specific abundance when planning experiments.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers and research laboratories with premium IVD raw materials, covering every stage from concept to clinic. The PPP1R3B antibody supports research into glycogen metabolism disorders, diabetes, and insulin signaling pathways. For your assay development, CamelBio offers validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and expert sourcing for rare targets—ensuring reliable supply for your diagnostic needs.