Anti-PPP1R14B Rabbit Polyclonal Antibody for WB, ELISA - Q96C90

Core Product Specifications and Parameters

Biological Background: PPP1R14B Function and Localization

• PPP1R14B, also known as Phospholipase C-beta-3 neighbouring gene protein (PNG), is a protein phosphatase 1 (PP1) regulatory subunit that inhibits the catalytic subunit PPP1CA.
• Its inhibitory activity on PP1 is increased more than 50-fold upon phosphorylation, indicating a phosphorylation-dependent regulatory mechanism.
• The protein localizes to the cytoplasm, consistent with its role in modulating phosphatase activity within cellular signaling pathways.
• PPP1R14B is ubiquitously expressed at low levels across tissues, suggesting a housekeeping regulatory function in diverse cell types.
• Post-translational modifications include phosphorylation and acetylation, which may further fine-tune its activity and interactions.
• The protein contains a coiled coil domain, which may mediate protein-protein interactions essential for its regulatory function.

Experimental Guidance and Technical Tips

• For Western blot analysis, expect a band at approximately 16 kDa. Use high-percentage SDS-PAGE gels or low molecular weight markers for optimal resolution of this small protein.
• The immunogen corresponds to amino acids 68–147, so validate detection specificity in your sample system, especially for isoforms or post-translationally modified forms.
• This antibody is suitable for ELISA; titrate the antibody to achieve the best signal-to-noise ratio for your assay conditions.
• Given its polyclonal nature, lot-to-lot variability may occur. Consider re-validating the antibody if switching lots for critical experiments.
• Explore phosphorylation-dependent binding by performing phosphatase treatment or using phospho-mimetic conditions to control PP1 inhibition.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides comprehensive IVD raw material solutions and technical support to accelerate diagnostic development. This anti-PPP1R14B polyclonal antibody supports research into phosphatase signaling, and CamelBio can further supply validated antibody pairs, recombinant proteins, and ancillary reagents to streamline your IVD assay pipeline. Contact us for customized sourcing of rare or challenging targets.