Anti-PPP1R14A (CPI-17) Rabbit Polyclonal Antibody for WB and ELISA - Q96A00

Core Product Specifications and Parameters

Biological Background: PPP1R14A Function and Localization

• Protein phosphatase 1 regulatory subunit 14A (PPP1R14A), also known as CPI-17, is a potent inhibitor of the catalytic subunit of protein phosphatase 1 (PPP1CA).
• Phosphorylation of CPI-17 enhances its inhibitory activity over 1000-fold, functioning as a molecular switch that modulates PPP1CA substrate phosphorylation and thereby regulates smooth muscle contraction.
• The protein exists in at least two isoforms generated by alternative splicing; isoform 1 is detected in aorta and testis, while isoform 2 is found in aorta.
• PPP1R14A localizes to the cytoplasm, consistent with its role in regulating cytosolic phosphatase activity.
• As a phosphoprotein, its activity is likely controlled by kinases such as protein kinase C (PKC), positioning it as a key node in signal transduction pathways governing muscle tone.
• Post-translational phosphorylation and its phosphatase inhibitor activity underscore its importance in proteomics and cellular signaling research.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant fusion protein encompassing the full-length human PPP1R14A (aa 1–147). Given this, the antibody is raised against the entire protein and may detect both phosphorylated and unphosphorylated forms; use phosphatase inhibitors in sample preparation if the phospho-state is relevant.
• For Western blotting, a band at approximately 17 kDa is expected. Consider using appropriate positive controls such as smooth muscle tissue lysates (e.g., aorta) or cell lines expressing PPP1R14A.
• In ELISA applications, the antibody can be employed for direct or indirect detection, but optimal working dilutions must be determined experimentally. Pairing with a specific anti-CPI-17 detection antibody may improve sensitivity.
• Cross-reactivity with mouse and rat is reported, facilitating translational studies in rodent models; however, validation in the relevant species and tissue is recommended before large-scale experiments.
• As a rabbit polyclonal antibody, minor lot-to-lot variations can occur; include a standardized control sample in each experiment to ensure consistency.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a trusted one-stop provider of IVD raw materials, supporting diagnostic manufacturers and research laboratories with high-quality antibodies, validated antibody pairs, and bulk reagents. For smooth muscle biology and phosphatase signaling research, this anti-PPP1R14A (CPI-17) antibody is part of a broader portfolio that includes optimized monoclonal and polyclonal antibodies targeting PPP1 family members. Together with our ancillary raw material supply and sourcing capabilities, CamelBio helps streamline assay development from concept to clinic.