Anti-PPP1R12B Rabbit Polyclonal Antibody for WB, ELISA - O60237

Core Product Specifications and Parameters

Biological Background: PPP1R12B Function and Localization

• PPP1R12B, also known as MYPT2 or PP1bp55, is the myosin phosphatase-targeting subunit 2 that forms a complex with protein phosphatase 1 (PP1).
• It critically regulates myosin phosphatase activity, thereby modulating the phosphorylation state of myosin light chains and controlling actomyosin contractility.
• PPP1R12B belongs to the MYPT family of myosin phosphatase-targeting subunits, distinct from MYPT1 (PPP1R12A) and MYPT3 (PPP1R12C), and contains ANK repeats important for protein-protein interactions.
• The protein localizes to the cytoplasm, specifically associated with the cytoskeleton and stress fibers, where it influences cytoskeletal dynamics and cell motility.
• Alternative splicing yields multiple isoforms; notably, isoforms 3 and 4 are heart-specific, suggesting specialized roles in cardiac muscle contractility.
• PPP1R12B is phosphorylated (as indicated by the keyword Phosphoprotein), which may regulate its activity in response to signals like Ca2+ and kinases.
• Tissue expression is broad, detected in skeletal muscle, heart, brain, placenta, kidney, spleen, thymus, pancreas, and lung.

Experimental Guidance and Technical Tips

• The immunogen spans residues 660–930, which lies in the C-terminal region; consider this epitope coverage when designing experiments to detect specific isoforms or truncation variants.
• For Western blot, the predicted molecular weight is approximately 110 kDa; use an appropriate gel percentage (e.g., 8–10% SDS-PAGE) for effective separation.
• Validate antibody specificity and sensitivity in relevant human tissue lysates or cell lines (e.g., heart or skeletal muscle) before large-scale application.
• For ELISA, optimize coating and detection conditions using the immunogen or purified protein as a positive control.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines your IVD raw-materials procurement by offering this anti-PPP1R12B antibody together with validated antibody pairs, bulk ancillary reagents, and rare-target sourcing in the field of cytoskeletal and contractility research. Our one-stop platform supports diagnostic manufacturers and laboratories from concept to clinic, ensuring a reliable supply chain for critical raw materials and technical expertise.