Anti-PPP1R12A Polyclonal Antibody for WB, IHC-P, ELISA - O14974

Core Product Specifications and Parameters

Biological Background: PPP1R12A Function and Localization

• PPP1R12A encodes protein phosphatase 1 regulatory subunit 12A, also known as myosin phosphatase-targeting subunit 1 (MYPT1), a crucial regulatory component of the myosin phosphatase complex.
• It serves as a key regulator of the catalytic subunit of protein phosphatase 1 (PPP1C), mediating its binding to myosin and targeting the phosphatase to specific substrates.
• Through its association with PPP1C, PPP1R12A participates in the dephosphorylation of Plk1, influencing cell cycle regulation.
• PPP1R12A is capable of inhibiting HIF1AN-dependent suppression of HIF1A activity, thereby modulating hypoxia-inducible factor signaling.
• The protein localizes predominantly to the cytoplasm and is associated with the actin cytoskeleton, particularly stress fibers, consistent with its role in actomyosin regulation.
• Tissue expression is notable in striated muscles, with specific enrichment in type 2a fibers, underscoring its importance in muscle contractile function.
• Structural features include multiple ankyrin (ANK) repeats and the presence of alternative splicing isoforms, hydroxylation sites, and multiple phosphorylation events (as indicated by keyword annotations).
• Disease-associated variants of PPP1R12A have been documented, linking the protein to human pathologies.

Experimental Guidance and Technical Tips

• The antibody was raised against a recombinant fragment spanning amino acids 710-970 of human PPP1R12A. Consider this region for epitope recognition when designing detection strategies.
• For Western blotting, a band of approximately 115 kDa is expected in lysates from human, mouse, and rat tissues/cells.
• IHC-P application may require antigen retrieval optimization; validate in relevant tissue sections known to express PPP1R12A, such as striated muscle.
• ELISA can be utilized for quantitative measurement; cross-reactivity with mouse and rat suggests utility across these species.

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CamelBio offers a seamless gateway to premium IVD raw materials, including this anti-PPP1R12A polyclonal antibody designed for myosin phosphatase and cytoskeletal research. Our portfolio encompasses validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target sourcing, supporting diagnostic manufacturers and laboratories from concept to clinic.