Anti-PPP1R12A Polyclonal Antibody for WB, IF/ICC, IP, ELISA - O14974

Core Product Specifications and Parameters

Biological Background: PPP1R12A Function and Localization

• PPP1R12A (MYPT1) is a regulatory subunit that targets protein phosphatase 1C (PPP1C) to myosin, regulating myosin light chain phosphorylation.
• Mediates the dephosphorylation of PLK1 as part of the PPP1C complex, contributing to mitotic regulation.
• Inhibits HIF1AN-dependent suppression of HIF1A, potentially impacting hypoxia response pathways.
• Localizes to the cytoplasm and stress fibers, consistent with its role in actomyosin contractility.
• Predominantly expressed in striated muscles, with specific enrichment in type 2a fibers.
• Undergoes post-translational modifications including hydroxylation and phosphorylation, which may modulate its activity.
• Contains ANK repeat domains, characteristic of protein-protein interaction modules.

Experimental Guidance and Technical Tips

• For Western blotting, the predicted molecular weight is ~115 kDa; ensure adequate protein transfer and use appropriate markers.
• For immunoprecipitation, the antibody's reactivity with human, mouse, and rat samples makes it suitable for cross-species studies; validate in the relevant sample system.
• In immunofluorescence/immunocytochemistry (IF/ICC), the localization to cytoplasm and stress fibers may be observed; consider fixation/permeabilization conditions that preserve cytoskeletal structures.
• Standard ELISA optimization is recommended, including checkerboard titrations to determine optimal coating and antibody concentrations.
• The immunogen corresponds to the N-terminal region (aa 1-200), which may be conserved across species and isoforms; verify sequence homology if extending to other species not tested.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive range of IVD raw materials, including validated antibody pairs, monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target sourcing. Our PPP1R12A antibody supports research into phosphatase regulation, muscle contractility, and cytoskeletal dynamics. With one-stop access from concept to clinic, CamelBio empowers diagnostic manufacturers and labs to accelerate assay development.