Anti-PPP1CA Rabbit Polyclonal Antibody for WB - P62136

Core Product Specifications and Parameters

Biological Background: PPP1CA Function and Localization

• Serine/threonine-protein phosphatase PP1-alpha catalytic subunit (PPP1CA) is the catalytic component of Protein Phosphatase 1 (PP1) holoenzymes, which associate with over 200 regulatory proteins to achieve substrate specificity and direct dephosphorylation of hundreds of targets. Related references: PMID:28216226 PMID:30158517 PMID:35768504
• PP1, containing PPP1CA, is essential for fundamental cellular processes including cell division, transcription elongation, glycogen metabolism, muscle contractility, and protein synthesis. Related references: PMID:35768504 PMID:35830882 PMID:35831509
• PPP1CA acts as a catalytic component of the PNUTS-PP1 complex, promoting RNA polymerase II transcription pause-release and elongation by dephosphorylating key transcription-associated factors such as AFF4, CDK9, and SUPT5H. Related references: PMID:39603239 PMID:39603240 PMID:31677974
• It plays a role in the control of chromatin structure and cell cycle progression during the transition from mitosis into interphase. Related references: PMID:20516061
• PPP1CA contributes to circadian rhythm regulation by balancing CSNK1D and CSNK1E activity, thereby modulating the phosphorylation speed and rhythmicity of PER1 and PER2. Related references: PMID:21712997
• As a core component of the SHOC2-MRAS-PP1c (SMP) holophosphatase complex, PPP1CA dephosphorylates inhibitory serine residues on RAF kinases, thus stimulating MAPK pathway activity. Related references: PMID:35768504 PMID:35830882 PMID:35831509 PMID:36175670
• Localization includes cytoplasm, nucleus, nucleoplasm, nucleolus, and postsynaptic density, reflecting its involvement in diverse cellular and neuronal functions.
• Key features include acetylation, alternative splicing, phosphoprotein status, manganese binding, and a role in host-virus interactions (necessary for alphavirus replication).

Experimental Guidance and Technical Tips

• For Western blotting, expect a band at approximately 38 kDa; validate with appropriate positive and negative controls in your sample system.
• The immunogen (amino acids 230-330) is highly conserved among human, mouse, and rat, which supports the observed cross-reactivity; sequence alignment should be reviewed if other species are tested.
• Due to nuclear and cytoplasmic localization, optimize fixation and permeabilization conditions for immunofluorescence or ICC.
• Consider co-immunoprecipitation or complex-specific detection methods if studying PP1 holoenzyme interactions, as PPP1CA partners with numerous regulatory subunits.
• Always titrate antibody dilution for ELISA and confirm specificity with a suitable blocking strategy.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive portfolio of IVD raw materials, including validated antibodies, antigens, and ancillary reagents, supporting every stage from concept to clinic. This PPP1CA antibody is a reliable tool for research into phosphatase signaling, cell cycle regulation, and metabolic pathways. Leverage our one-stop procurement solutions for seamless access to rare-target raw materials, bulk consumables, and technical support, all tailored to your diagnostic development needs.