Anti-PPP1CA Rabbit Monoclonal Antibody for WB, IF/ICC, ELISA - P62136

Core Product Specifications and Parameters

Biological Background: PPP1CA (PP1-alpha catalytic subunit) Function and Localization

• PPP1CA encodes the serine/threonine-protein phosphatase PP1-alpha catalytic subunit, a member of the PPP family of protein phosphatases. It associates with over 200 regulatory subunits to form substrate-specific holoenzymes. Related references: PMID:28216226, PMID:30158517, PMID:35768504
• PP1 is indispensable for cell division, transcription elongation, glycogen metabolism, muscle contractility, and protein synthesis. Related references: PMID:35768504, PMID:35830882, PMID:35831509
• As the catalytic subunit of the PNUTS-PP1 complex, PP1-alpha promotes RNA polymerase II pause-release and transcription elongation, regulates transcription termination, and mediates the response to replication stress. Related references: PMID:39603239, PMID:39603240, PMID:31677974, PMID:33264625
• The protein localizes to the cytoplasm, nucleus (nucleoplasm and nucleolus), and postsynaptic density.
• PP1-alpha helps determine circadian period length by regulating the phosphorylation speed of PER1 and PER2, in balance with CSNK1D and CSNK1E. Related references: PMID:21712997
• It dephosphorylates FOXP3 in Treg cells (rheumatoid arthritis), ATG16L1 (inhibiting autophagy), and RIG-I/IFIH1 (activating innate immune response). Related references: PMID:23396208, PMID:26083323, PMID:23499489
• PP1-alpha is a core component of the SHOC2-MRAS-PP1c (SMP) holophosphatase complex, which dephosphorylates and activates RAF1, BRAF, and ARAF kinases, thereby regulating the MAPK pathway. Related references: PMID:35768504, PMID:35830882, PMID:35831509, PMID:36175670
• The protein is necessary for alphavirus replication (host-virus interaction).

Experimental Guidance and Technical Tips

• The immunogen was designed against a synthetic peptide within amino acids 230-330 of human PPP1CA, a region conserved across human, mouse, and rat, which likely contributes to the reported cross-reactivity.
• For WB, the observed band is ~38 kDa; loading controls and appropriate blocking conditions should be optimized experimentally.
• For IF/ICC, fixation and permeabilization protocols should be validated in the target cell line, as nuclear and cytoplasmic localization may require specific conditions.
• ELISA detection may be used for quantifying PPP1CA in lysates, but standard curves and assay conditions need to be established by the end user.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your comprehensive partner for IVD raw-material procurement, from validated antibody pairs and optimized monoclonal antibodies to bulk ancillary reagents and rare target sourcing. This PPP1CA antibody supports research in cell cycle, transcription regulation, and signal transduction, with CamelBio’s integrated supply chain ensuring reliable and scalable access for your diagnostic and research needs.