Anti-PPP1CA Polyclonal Antibody for WB, IF/ICC, ELISA - P62136

Core Product Specifications and Parameters

Biological Background: PPP1CA Function and Localization

• PPP1CA encodes the catalytic subunit of serine/threonine-protein phosphatase PP1-alpha, which associates with over 200 regulatory proteins to form holoenzymes that dephosphorylate hundreds of biological targets. Related references: PMID:28216226, PMID:30158517, PMID:35768504
• PP1 is essential for cell division, transcription elongation, glycogen metabolism, muscle contractility, and protein synthesis. Related references: PMID:35768504, PMID:35830882, PMID:35831509
• As part of the PNUTS-PP1 complex, it promotes RNA polymerase II transcription pause-release by dephosphorylating AFF4, CDK9, and other factors; also regulates transcription termination and chromatin structure during mitosis-to-interphase transition. Related references: PMID:39603239, PMID:39603240, PMID:31677974, PMID:33264625, PMID:20516061
• The SHOC2-MRAS-PP1c (SMP) complex specifically dephosphorylates RAF1, BRAF, and ARAF kinases at inhibitory sites, activating the MAPK pathway. Related references: PMID:35768504, PMID:35830882, PMID:35831509, PMID:36175670
• It dephosphorylates FOXP3 in Treg cells, impacting immune function; dephosphorylates CENPA; and inhibits autophagy by dephosphorylating ATG16L1. Related references: PMID:23396208, PMID:25556658, PMID:26083323
• Along with PPP1CC, it activates RIG-I and MDA5 by dephosphorylation, contributing to innate immune response. Related references: PMID:23499489
• It localizes to the cytoplasm, nucleus, nucleoplasm, nucleolus, and postsynaptic density.
• It plays roles in circadian rhythm regulation by opposing CSNK1D/E kinases on PER proteins. Related references: PMID:21712997

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 1-330 of human PPP1CA, covering the entire N-terminal domain; the resulting polyclonal antibody is expected to recognize multiple epitopes.
• For western blot, the mature protein migrates at approximately 38kDa; confirm band identity using positive and negative control lysates from human, mouse, or rat tissues.
• In immunofluorescence/ICC, optimize fixation and permeabilization protocols based on the cellular compartment of interest (nucleus/nucleolus/cytoplasm); include appropriate secondary-only controls.
• ELISA applications may be useful for quantifying PPP1CA levels or assessing immunogen reactivity; validate linearity and sensitivity using known standards.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing partner for IVD raw materials, providing diagnostic manufacturers and labs with validated antibodies, antigen pairs, and ancillary reagents for phosphatase and signaling research. We support assay development from concept to clinic with bulk supply options and rare-target sourcing. Contact us to streamline your procurement of high-quality raw materials for PPP1CA and other key targets.