Anti-PPME1 Polyclonal Antibody for WB and ELISA - Q9Y570

Core Product Specifications and Parameters

Biological Background: PPME1 Function and Localization

• PPME1, also known as PME-1, is a protein phosphatase methylesterase that specifically demethylates the catalytic subunits of protein phosphatase 2A (PP2A), including PPP2CA and PPP2CB.
• It functions as a serine esterase and hydrolyzes the methyl ester bond on the C‑terminal leucine residue of PP2A catalytic subunits, reversing their reversible carboxymethylation.
• Demethylation of PP2A by PPME1 leads to displacement of the manganese ion from the active site and consequent inactivation of the phosphatase, providing a critical regulatory mechanism for PP2A‑dependent signaling.
• PPME1 is classified under the AB hydrolase superfamily and is a key modulator of methylation‑dependent signaling pathways; it is also identified as an alternative splicing variant and phosphoprotein.
• It has been characterized by proteomics identification and is part of the reference proteome for human; structural studies have revealed the molecular basis for its interaction with PP2A.

Experimental Guidance and Technical Tips

• The immunogen used corresponds to the N‑terminal region (amino acids 1–160) of human PPME1; this region may be critical for detecting the full‑length protein and for discriminating specific isoforms or cleavage products.
• For Western blot (WB), it is advisable to validate the antibody in appropriate lysates (e.g., from human, mouse, and rat cell lines or tissues) and consider the predicted molecular weight of ~42 kDa, though post‑translational modifications may alter migration.
• In ELISA applications, the antibody may be suitable for direct or sandwich formats; preliminary checkerboard titrations are recommended to optimize coating and detection conditions.
• Since PPME1 regulates PP2A methylation, the antibody can be useful in studies investigating PP2A signaling, cancer biology, or neurological disorders, but specificity should be confirmed using appropriate controls (e.g., knockdown or knockout lysates).

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic manufacturers and research teams by providing one‑stop access to high‑quality IVD raw materials and technical support, from concept to clinic. Our portfolio includes validated antibody pairs, optimized polyclonal and monoclonal antibodies, bulk ancillary reagents, and sourcing for rare‑target raw materials. For researchers focused on protein methylation and PP2A biology, we offer reliable PPME1 antibodies and related reagents to accelerate your assay development and production needs.