Anti-PPM1J Rabbit Polyclonal Antibody for WB and ELISA - Q5JR12

Core Product Specifications and Parameters

Biological Background: PPM1J Function and Localization

• PPM1J (Protein Phosphatase 1J) is a serine/threonine phosphatase belonging to the PP2C family, annotated as a hydrolase and protein phosphatase in UniProt.
• The protein is also known as PP2C-zeta, with alternative names including PP2CZ and PPP2CZ.
• PPM1J undergoes alternative splicing, producing multiple isoforms that may have distinct functions or expression patterns.
• The canonical isoform has a calculated molecular weight of approximately 54.8 kDa.
• According to UniProt keywords, PPM1J is a phosphoprotein, suggesting it may be regulated by phosphorylation events.
• PPM1J has been identified through proteomics identification, confirming its protein expression in human cells.
• As part of the reference proteome, the PPM1J sequence is well-annotated and reliable for research purposes.

Experimental Guidance and Technical Tips

• The immunogen (aa 130–260) is an internal region; consider validating the antibody’s reactivity in your target species beyond human and mouse if needed.
• For Western blot, a band near 55 kDa is expected; include appropriate positive and negative controls to confirm specificity.
• In ELISA, optimize coating antigen and antibody titration for your assay format.
• As a polyclonal antibody, lot-to-lot consistency may vary; re-validate each new lot in your system.
• Due to alternative splicing, verify detection of the desired isoform(s) using isoform-specific controls if necessary.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, laboratories, and research institutions a streamlined procurement pathway for IVD raw materials, including antibodies against key signaling targets like PPM1J. Our catalog features validated polyclonal and monoclonal antibodies, ancillary reagents, and access to rare target sourcing—all supported by technical consultation to bridge the gap from concept to clinical application. Let CamelBio be your reliable partner in phosphatase-related research and beyond.