Anti-PPM1F Rabbit Polyclonal Antibody for WB, ELISA - P49593

Core Product Specifications and Parameters

Biological Background: PPM1F Function and Localization

• PPM1F, also known as CaM-kinase phosphatase (CaMKPase) or hFem-2, is a protein phosphatase that belongs to the PP2C family.
• It functions primarily to dephosphorylate and deactivate CaM-kinases II, IV, and I, which are activated by autophosphorylation or upstream kinases, thereby negatively regulating calcium/calmodulin-dependent signaling pathways.
• The enzyme promotes apoptosis, indicating a role in programmed cell death.
• PPM1F requires divalent metal cations such as magnesium or manganese for its catalytic activity.
• Alternative splicing generates multiple isoforms of PPM1F, which may exhibit distinct regulatory properties.
• The protein has been identified in proteomics studies, confirming its expression in various human tissues.

Experimental Guidance and Technical Tips

• The immunogen used for this polyclonal antibody corresponds to the N-terminal region (amino acids 1-190) of human PPM1F, ensuring targeted recognition of this functional domain.
• For Western blot (WB), consider using whole cell lysates or tissue extracts from human samples, with a recommended initial dilution factor to be optimized in your system.
• The protein has a predicted molecular weight of approximately 50 kDa; however, due to potential post-translational modifications or isoform variations, the observed band may shift. Validate the specific band with appropriate controls.
• For ELISA applications, optimize the coating antigen concentration and antibody dilution to achieve the best signal-to-noise ratio.
• Cross-reactivity with other species is not reported; thus, it is advised to validate reactivity if using non-human samples.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio partners with diagnostic manufacturers and research laboratories to provide a comprehensive range of IVD raw materials, including this anti-PPM1F antibody for apoptosis and cell signaling investigations. From validated antibody pairs to bulk ancillary reagents, we streamline procurement for your assay development needs. Our expert team supports sourcing even rare-target reagents, ensuring you have reliable materials from concept to clinic.