Anti-PPM1E Rabbit Monoclonal Antibody for WB, ELISA - Q8WY54

Core Product Specifications and Parameters

Biological Background: PPM1E Function and Localization

• PPM1E (Protein phosphatase 1E), also known as POPX1, CaMKP-N, CaMKN, or CAMKPN, is a member of the PP2C family of Ser/Thr protein phosphatases.
• Encoded by the PPM1E gene in humans (GeneID: 22843), it functions as a protein phosphatase that inactivates multifunctional CaM kinases such as CAMK4 and CAMK2.
• Additionally, PPM1E dephosphorylates and inactivates PAK, and may play a role in regulating actin fiber stress breakdown and morphological changes driven by TNK2/CDC42 signaling.
• It also dephosphorylates PRKAA2 (AMPK α2), implicating it in energy-sensing pathways.
• PPM1E localizes to both the nucleus and cytoplasm, indicating a dynamic role in multiple cellular compartments.
• The protein harbors metal-binding sites (Mg²+ or Mn²+) essential for its phosphatase activity, and undergoes alternative splicing.
• Post-translational modifications: identified as a phosphoprotein via proteomics.
• Predicted molecular weight: ~84 kDa, matching the calculated mass of 83952 Da.

Experimental Guidance and Technical Tips

• The immunogen was a synthetic peptide corresponding to amino acids 500–600 of human PPM1E. For Western blot, a band around 84 kDa is expected in human cell lysates. Consider using appropriate positive and negative controls.
• For ELISA applications, optimize coating conditions and antibody concentrations. Validate specificity with recombinant PPM1E or peptide competition assays.
• Note that the antibody is reactive with human samples; cross-reactivity with other species has not been tested. Confirm suitability if working with non-human samples.
• Because PPM1E is a phosphatase that regulates CAMK and PAK pathways, consider stimulating cells to modulate its activity or expression for functional studies.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—from concept to clinic. This anti-PPM1E antibody supports research into kinase/phosphatase signaling, cancer cell motility, and metabolic regulation. Beyond monoclonal antibodies, CamelBio offers validated antibody pairs, optimized bulk reagents, and rare target sourcing to streamline your assay development and manufacturing workflows.