Anti-PPM1A Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P35813

Core Product Specifications and Parameters

Biological Background: PPM1A Function and Localization

• PPM1A (Protein phosphatase 1A), also known as PP2Cα or PP2CA, is a serine/threonine phosphatase encoded by the PPM1A gene in humans. It belongs to the PP2C family of metal-dependent protein phosphatases.
• It functions as a broad-specificity enzyme that negatively regulates TGF-beta signaling by dephosphorylating SMAD2 and SMAD3, leading to their dissociation from SMAD4 and nuclear export, thereby terminating the signal.
• PPM1A plays a critical role in dampening TNF-mediated NF-kappa-B activation by dephosphorylating and inactivating IKBKB/IKKβ kinase.
• It also dephosphorylates AMPK subunits PRKAA1 and PRKAA2, implicating it in energy homeostasis pathways.
• Subcellularly, PPM1A is found in the nucleus, cytoplasm, cytosol, and membrane-associated compartments, consistent with its diverse signaling roles.
• The protein undergoes alternative splicing, is myristoylated, and is itself phosphorylated. It requires magnesium or manganese ions as cofactors for catalytic activity.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (aa 161-370) of human PPM1A, which may confer specificity for the canonical PP2Cα isoform; verify performance in the relevant sample system.
• For Western blot (WB), the predicted molecular weight is approximately 42 kDa; consider loading appropriate positive controls (e.g., HeLa or HEK293 lysates) and optimizing blocking conditions.
• For immunofluorescence/ICC, permeabilization and fixation methods may influence detection of nuclear and cytoplasmic pools; validate co-localization markers.
• When used in ELISA, the antibody may be suitable as both capture and detection reagent; perform cross-titration to determine optimal working concentrations.
• As cross-reactivity is confirmed for human, mouse, and rat, species-specific signal should be validated with endogenous tissue extracts or cell lines.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a comprehensive IVD raw-material sourcing partner, offering validated antibody pairs, optimized mono- and polyclonal antibodies, bulk ancillary reagents, and custom rare-target material procurement. This PPM1A polyclonal antibody supports phosphatase signaling, TGF-beta, and NF-kB pathway research and assay development. Let CamelBio streamline your supply chain from discovery to clinical application.