Anti-PPM1A Monoclonal Antibody for WB, ELISA - P35813

Core Product Specifications and Parameters

Biological Background: PPM1A Function and Localization

• PPM1A (Protein Phosphatase 1A), also known as PP2C-alpha or PP2CA, is a member of the PP2C family of serine/threonine phosphatases that dephosphorylates a broad range of substrates.
• It negatively regulates TGF-beta signaling by dephosphorylating SMAD2 and SMAD3, leading to their dissociation from SMAD4, nuclear export, and signal termination.
• PPM1A plays a critical role in terminating TNF-mediated NF-kappa-B activation through dephosphorylation and inactivation of IKBKB/IKK-beta.
• The protein localizes to multiple cellular compartments including the nucleus, cytoplasm, cytosol, and membrane-associated regions, indicating versatile regulatory functions.
• PPM1A undergoes alternative splicing and post-translational modifications such as myristoylation and phosphorylation, which may influence its activity and localization.

Experimental Guidance and Technical Tips

• The immunogen used is a recombinant fusion protein covering amino acids 1-382 of human PPM1A (NP_066283.1), which may detect full-length protein and potentially some isoforms; consider verifying specificity in your target sample system.
• For Western blot (WB) applications using human cell lysates, the expected band is around 42 kDa; validate using appropriate positive and negative controls.
• ELISA utility has been reported; when setting up detection assays, optimize coating antigen and antibody concentrations to achieve desired sensitivity.
• Because PPM1A is involved in multiple signaling pathways, consider using pathway-specific activators/inhibitors to test antibody performance under different biological conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with a reliable, integrated supply chain for IVD raw materials. This anti-PPM1A monoclonal antibody exemplifies our capability to deliver validated antibodies for key signaling targets. Beyond single antibodies, we offer matched antibody pairs, bulk ancillary reagents, and sourcing support for rare-target raw materials, accelerating your transition from research to clinical assay development.