Anti-PPL Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - O60437

Core Product Specifications and Parameters

Biological Background: Periplakin Function and Localization

• Periplakin (PPL) is a structural component of the cornified envelope, a specialized layer beneath the plasma membrane of terminally differentiated keratinocytes.
• It may link the cornified envelope to desmosomes and intermediate filaments, providing mechanical integrity to stratified epithelia.
• PPL has been suggested to act as a localization signal in PKB/AKT-mediated signaling pathways, potentially influencing cell survival and metabolism.
• The protein belongs to the plakin family of cytolinkers, characterized by multiple coiled-coil regions and an SH3 domain.
• Subcellularly, PPL is localized to desmosomes (cell junctions), the cytoplasm, cytoskeleton, and lateral cell membranes.
• Tissue specificity is prominent in stratified squamous epithelia, with expression also detected in some other epithelial tissues.
• Post-translational modifications include phosphorylation, and the protein is known to be a phosphoprotein.
• Functional keywords include keratinization, cell junction, membrane, and cytoskeleton, reflecting its role in epidermal barrier formation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1-130) of human PPL; consider epitope accessibility in native versus denaturing conditions.
• For Western blot (WB), a band around the predicted ~205 kDa may be observed; validate using appropriate positive controls such as human keratinocyte lysates.
• In immunofluorescence/immunocytochemistry (IF/ICC), anticipate labeling at cell-cell junctions and membrane regions in stratified epithelial cell lines.
• Species cross-reactivity is reported for human, mouse, and rat; however, we recommend validating in the specific sample system.
• ELISA performance should be confirmed by the end-user with the appropriate recombinant protein or cell lysates.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive IVD raw-material partner, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents to support your diagnostic development. For targets like periplakin, we can assist in sourcing rare raw materials and provide tailored technical consultation to streamline your cornified envelope or epithelial research. Let CamelBio bridge your journey from concept to clinic with reliable, high-quality products.