Anti-PPIF Rabbit Polyclonal Antibody for WB and ELISA - P30405

Core Product Specifications and Parameters

Biological Background: PPIF Function and Localization

• PPIF encodes Cyclophilin D (CypD), also known as cyclophilin F or mitochondrial cyclophilin (CyP-M), a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family.
• It catalyzes cis-trans isomerization of proline imidic peptide bonds, aiding protein folding, as demonstrated in oligopeptide assays (PubMed:20676357). Related references: PMID:20676357
• CypD is a key regulator of the mitochondrial permeability transition pore (mPTP); it binds and promotes the open probability of mPTP, potentially masking the inhibitory phosphate binding site, a role that may be independent of its PPIase activity (PubMed:26387735). Related references: PMID:26387735
• In cooperation with mitochondrial p53, CypD triggers oxidative stress-induced necrosis, linking mitochondrial dysfunction to cell death (PubMed:22726440). Related references: PMID:22726440
• It modulates F(1)F(0) ATP synthase activity and regulates mitochondrial adenine nucleotide levels, impacting energy metabolism (By similarity).
• CypD also exhibits anti-apoptotic activity: it cooperates with BCL2 to inhibit cytochrome c-dependent apoptosis, a function independent of its mPTP regulation (PubMed:19228691). Related references: PMID:19228691
• The protein localizes to the mitochondrial matrix, consistent with its roles in mitochondrial function and apoptosis.
• Post-translational modifications include acetylation and S-nitrosylation; alternative splicing generates multiple isoforms.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 100-207 of human PPIF (NP_005720.1), covering a region critical for PPIase activity; consider validating specificity in the relevant species (human, mouse, rat) using Western blotting.
• For WB, a band at approximately 22 kDa is expected; optimize loading and antibody dilution for clear detection.
• In ELISA, ensure proper coating and blocking conditions; cross-reactivity with other cyclophilins may need to be assessed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supplies this anti-PPIF rabbit polyclonal antibody as part of our comprehensive IVD raw-material portfolio, supporting research into mitochondrial biology, apoptosis, and necrosis. We provide validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents, facilitating seamless procurement for diagnostic development and research applications.