Anti-PPIE Rabbit Polyclonal Antibody for WB, IP, ELISA - Q9UNP9

Core Product Specifications and Parameters

Biological Background: PPIE Function and Localization

• PPIE, also known as Cyclophilin E or Cyclophilin-33, is a peptidyl-prolyl cis-trans isomerase that belongs to the cyclophilin family. Multiple isoforms are produced by alternative splicing.
• It is a component of the spliceosome and plays a role in pre-mRNA splicing. Related references: PMID:11991638 PMID:28076346
• PPIE combines RNA-binding and PPIase activities, binding single-stranded RNA with preference for poly-A and poly-U stretches, likely targeting the poly(A)-region of mRNA 3'-UTRs. Related references: PMID:18258190 PMID:20460131 PMID:20677832 PMID:8977107
• It catalyzes the cis-trans isomerization of proline imidic peptide bonds in proteins, which is essential for its function. Related references: PMID:18258190 PMID:20541251 PMID:20677832
• PPIE inhibits KMT2A activity in a proline isomerase-dependent manner. Related references: PMID:20460131 PMID:20541251 PMID:20677832
• The protein is predominantly localized in the nucleus, consistent with its role in mRNA processing.
• PPIE is ubiquitously expressed across various tissues including heart, brain, placenta, lung, liver, skeletal muscle, kidney, and pancreas.
• Post-translational modifications include phosphorylation, which may regulate its activity.

Experimental Guidance and Technical Tips

• The immunogen used is a recombinant fusion protein covering full-length human PPIE (amino acids 1-301). For optimal results in WB, consider using whole cell or nuclear extracts from human or mouse cells.
• For IP, validate antibody-antigen complex formation in the relevant sample system, as extraction conditions may affect epitope availability.
• ELISA may be used for detection of PPIE in solution; ensure antigen coating conditions are optimized for recombinant protein.
• Cross-reactivity with mouse suggests conservation of the epitope; perform sequence alignment to confirm similarity.
• Given PPIE's nuclear localization, fractionation protocols may enhance signal in WB.

CamelBio: Your One-Stop Sourcing Bridge

At CamelBio, we support diagnostic manufacturers, labs, and research institutes with comprehensive access to IVD raw materials. This Anti-PPIE antibody complements our portfolio of spliceosome and RNA-binding protein tools, and we offer bulk sourcing, validated antibody pairs, and customized development services for your workflow needs.