Anti-PPIC Rabbit Polyclonal Antibody for WB, ELISA - P45877

Core Product Specifications and Parameters

Biological Background: Peptidyl-prolyl cis-trans isomerase C Function and Localization

• Peptidyl-prolyl cis-trans isomerase C (PPIC), also known as cyclophilin C or rotamase C, belongs to the cyclophilin family of immunophilins that possess peptidyl-prolyl isomerase activity.
• PPIC catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides, a rate-limiting step in protein folding.
• The protein is primarily localized in the cytoplasm, consistent with its role in assisting intracellular protein folding.
• Tissue expression analysis reveals that PPIC is broadly expressed, with highest levels in kidney, skeletal muscle, pancreas, heart, lung, and liver, and lower expression in brain.
• Keywords associated with PPIC include 3D-structure, isomerase, rotamase, and reference proteome, indicating its well-characterized structural and functional properties.
• The calculated molecular weight of the mature PPIC protein is approximately 22.7 kDa.

Experimental Guidance and Technical Tips

• The immunogen spans the mature PPIC sequence (aa 23-212), which likely raises antibodies recognizing the native conformation. For Western blotting under denaturing conditions, consider linear epitope accessibility.
• For ELISA applications, the polyclonal nature may offer broad epitope coverage, potentially enhancing sensitivity in sandwich assays when paired with a monoclonal capture antibody.
• Validate antibody performance in the relevant sample system, considering the tissue-specific expression pattern of PPIC (e.g., kidney or skeletal muscle lysates).
• Due to the conserved cyclophilin domain, assess potential cross-reactivity with other cyclophilin family members if specificity is critical.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, supporting diagnostic manufacturers, laboratories, and research institutes from concept to clinic. For protein folding and isomerase research, CamelBio can supply validated antibody pairs, optimized polyclonal antibodies like this PPIC antibody, bulk ancillary reagents, and assist in sourcing rare targets. Our expertise streamlines the procurement of reliable raw materials for your assay development needs.