Anti-PPHLN1 Rabbit Polyclonal Antibody for WB and ELISA - Q8NEY8

Core Product Specifications and Parameters

Biological Background: PPHLN1 Function and Localization

• Periphilin-1 (PPHLN1, also known as CDC7 expression repressor CR, HSPC206, HSPC232, or gastric cancer antigen Ga50) is an RNA-binding protein and core component of the human silencing hub (HUSH) complex.
• PPHLN1 functions within the HUSH complex to epigenetically repress mobile genetic elements, including retroviruses and transposable elements, thereby safeguarding genome integrity. Related references: PMID:15474462, PMID:17963697, PMID:26022416
• The HUSH complex predominantly targets LINE-1 (L1) retrotransposons, where PPHLN1 binds nascent transcripts to initiate silencing and maintain HUSH at chromatin. Related references: PMID:32976585, PMID:39658355
• Through recruitment of the histone methyltransferase SETDB1 and chromatin remodeler MORC2, the HUSH complex promotes H3K9me3 deposition and chromatin compaction at target loci. Related references: PMID:26022416
• PPHLN1 also contributes to HUSH2 complex-mediated repression of interferon-stimulated genes and silencing of unintegrated retroviral DNA. Related references: PMID:30487602, PMID:33144593, PMID:39013473
• Although ubiquitously expressed, PPHLN1 has been implicated in epithelial differentiation and barrier formation. Related references: PMID:12853457
• Subcellularly, PPHLN1 localizes to the nucleus, cytoplasm, and chromosome, consistent with its transcriptional regulatory roles. Post-translational modifications include acetylation and phosphorylation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 195–255 of human PPHLN1; consider sequence alignment with mouse and rat orthologs to assess cross-reactivity specificity.
• For western blot, a band at approximately 53 kDa is expected; validate detection in human, mouse, and rat lysates under reducing conditions.
• This polyclonal antibody is suitable for ELISA; titrate the antibody and include appropriate negative controls to ensure signal specificity.
• When probing HUSH complex components, consider co-immunoprecipitation or chromatin immunoprecipitation workflows to complement functional studies.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw-material access with validated antibody pairs, optimized monoclonal and polyclonal antibodies, and ancillary reagents in bulk—supporting seamless progression from development to production. For research on epigenetic silencing and the HUSH complex, our portfolio includes thoroughly characterized antibodies like this anti-PPHLN1 polyclonal, coupled with sourcing capabilities for rare-target raw materials. Partner with CamelBio to simplify your supply chain and accelerate time to clinic.