Anti-PPFIBP1 Rabbit Polyclonal Antibody for WB, ELISA - Q86W92

Core Product Specifications and Parameters

Biological Background: PPFIBP1 Function and Localization

• PPFIBP1 encodes liprin-beta-1, a scaffold protein also known as L2, SGT2, hSGT2, hSgt2p, and NEDSMBA, which contains coiled-coil and repeat regions.
• Liprin-beta-1 may regulate the disassembly of focal adhesions but does not bind receptor-like tyrosine phosphatases type 2A.
• The protein localizes to the cytoplasm and cell cortex, consistent with a role in anchoring or organizing focal adhesion complexes.
• It is widely expressed across human tissues with the notable exception of liver, as documented in proteomics analyses.
• Post-translational modifications include acetylation, phosphorylation, and ubiquitination (isopeptide bond formation), suggesting tight regulatory control.
• Alternative splicing produces multiple isoforms, and disease-associated variants have been linked to epilepsy and intellectual disability.
• According to UniProt, the canonical molecular weight is 114,024 Da, matching the observed ~114 kDa band in Western blot.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (aa 846–1005), which may confer good specificity for long isoforms of PPFIBP1.
• For Western blot, a band around 114 kDa is expected; use lysates from human, mouse, or rat cell lines known to express PPFIBP1 (e.g., HeLa, NIH/3T3) as positive controls.
• Polyclonal antibodies can exhibit lot-to-lot variation; it is advisable to validate each new lot by testing against recombinant protein or knockdown samples.
• For ELISA, consider coating with the immunogen or a full-length liprin-beta-1 protein and titrating the antibody to determine optimal working concentration.
• Store the antibody as recommended; repeated freeze-thaw cycles may compromise activity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research groups by supplying a wide range of IVD raw materials, including validated primary antibodies like this anti-PPFIBP1 polyclonal antibody. Our portfolio spans monoclonal/polyclonal antibodies, recombinant antigens, and ancillary reagents needed for cell adhesion and neurology-related assays. For rare or customized targets, we leverage global sourcing to deliver one‑stop procurement from concept to clinic—helping you accelerate development with reliable, traceable materials.