Anti-PPFIA1 Polyclonal Antibody for WB, ELISA - Q13136

Core Product Specifications and Parameters

Biological Background: Liprin-alpha-1 Function and Localization

• Liprin-alpha-1 (PPFIA1, LIP-1) is an intracellular scaffold protein belonging to the liprin family; alternative splicing generates multiple isoforms.
• It contains coiled-coil domains and N-terminal sterile alpha motif (SAM) repeats, mediating protein-protein interactions.
• Liprin-alpha-1 may regulate focal adhesion disassembly, thereby influencing cell adhesion and migration.
• It interacts with receptor-like tyrosine phosphatases (LAR, PTPRF) and likely localizes them to specific plasma membrane regions, modulating extracellular signaling.
• Subcellularly, it is primarily cytoplasmic and enriched at the cell cortex.
• As a phosphoprotein, its function is likely regulated by phosphorylation.
• Ubiquitously expressed across tissues, indicating a general cellular role in adhesion and signaling.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a region (aa 660–800) within the central coiled-coil domain; the antibody may recognize multiple isoforms if this region is conserved.
• For Western blotting, consider using whole-cell lysates from human cell lines and validate a band near 136 kDa; additional bands may correspond to isoforms or differential phosphorylation.
• In ELISA applications, optimize coating conditions using the immunogen or recombinant liprin-alpha-1 as a standard.
• As a rabbit polyclonal antibody, lot-to-lot consistency may vary; include appropriate controls and consider lot screening for critical assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with integrated access to IVD raw materials, from concept to clinic. For studies on cell adhesion and tyrosine phosphatase signaling, we supply validated anti-PPFIA1 antibodies and can source bulk polyclonals, monoclonals, and ancillary reagents tailored to your assay development needs. Our portfolio includes customized sourcing of rare-target raw materials, backed by technical consultation to accelerate your project timelines.