Anti-PPCS Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - Q9HAB8

Core Product Specifications and Parameters

Biological Background: PPCS Function and Localization

• PPCS (Phosphopantothenate--cysteine ligase), also known as CMD2C or PPC synthetase, is a key enzyme in the coenzyme A (CoA) biosynthetic pathway. It catalyzes the ATP-dependent conjugation of cysteine to 4'-phosphopantothenate to form 4-phosphopantothenoylcysteine, the second step in CoA biosynthesis from vitamin B5. Related references: PMID:11923312 PMID:12906824 PMID:29754768
• The enzyme shows a preference for ATP over CTP as a cosubstrate, ensuring efficient utilization of cellular energy. Related references: PMID:11923312 PMID:12906824
• Genetic variants in PPCS have been associated with cardiomyopathy, highlighting its crucial role in cardiac energy metabolism.
• The protein undergoes post-translational modifications including acetylation, and is subject to alternative splicing, generating multiple isoforms.
• PPCS is classified as a ligase and contains an ATP-binding domain, consistent with its catalytic activity.

Experimental Guidance and Technical Tips

• The immunogen used to generate this antibody lies within amino acids 212–311 of human PPCS. Epitope mapping experiments may be performed using truncated constructs if precise binding site identification is required.
• For Western blotting, a band at the predicted molecular weight of 34 kDa is expected in human samples. Endogenous PPCS may also be detected; consider using appropriate controls.
• In immunohistochemistry (IHC-P), antigen retrieval conditions (such as pH and incubation time) may need optimization for each tissue type.
• ELISA applications may benefit from using the corresponding synthetic peptide as a positive control.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to high-quality IVD raw materials for targets like PPCS, involved in coenzyme A metabolism. Our portfolio includes validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. For rare targets or custom sourcing needs, our technical team bridges requirements from concept to clinic.