Anti-PPBP Polyclonal Antibody for WB, ELISA - P02775

Core Product Specifications and Parameters

Biological Background: PPBP Function and Localization

• PPBP (platelet basic protein), also known as CXCL7, is a secreted C-X-C motif chemokine that undergoes proteolytic processing to generate multiple functional variants, including connective tissue-activating peptide III (CTAP-III), beta-thromboglobulin (β-TG), and neutrophil-activating peptide 2 (NAP-2).
• LA-PF4, a cleavage product, stimulates DNA synthesis, mitosis, glycolysis, intracellular cAMP accumulation, and secretion of hyaluronic acid and sulfated glycosaminoglycan; it also promotes plasminogen activator formation in human synovial cells.
• NAP-2 acts as a ligand for the chemokine receptors CXCR1 and CXCR2, functioning as a potent chemoattractant and activator of neutrophils; NAP-2(1–63) is the most active form, while NAP-2(1–66), NAP-2(73), and NAP-2(74) retain activity.
• The antibacterial proteins TC-1 and TC-2 are released from activated platelet alpha-granules in vitro, contributing to innate defense.
• CTAP-III(1–81) is more potent than full-length CTAP-III at desensitizing chemokine-induced neutrophil activation.
• PPBP is secreted from platelets and localizes to the extracellular space, consistent with its roles in intercellular signaling and host defense.
• The protein contains characteristic chemokine features, including a disulfide-bonded structure, a secretory signal peptide, and a low molecular weight of approximately 14 kDa.
• As a multifunctional molecule, PPBP is implicated in cell proliferation, leukocyte recruitment, inflammation, and antimicrobial activity.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 31–128 of human PPBP, covering the mature secreted protein; the antibody may recognize full-length PPBP and its naturally occurring processed forms.
• For Western blot (WB) analysis, expect a band at approximately 14 kDa for the mature protein; additional smaller bands may appear due to proteolytic cleavage in platelet extracts or activated cells.
• Consider using platelet-rich plasma, recombinant PPBP/CXCL7, or supernatants from activated platelets as positive control samples.
• For ELISA applications, the polyclonal antibody can be used in either capture or detection settings; however, extensive validation for each specific isoform is recommended due to the cleavage complexity.
• The antibody is raised in rabbit and reacts with human PPBP; cross-reactivity with other species has not been characterized, so validation in the relevant experimental system is advised.
• Optimize antibody concentration and incubation conditions for your specific assay, as performance may vary with sample type and processing.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a dedicated sourcing partner, providing diagnostic developers and research laboratories with reliable access to high-quality IVD raw materials, such as this anti-PPBP polyclonal antibody. We specialize in streamlining procurement of validated antibodies, recombinant proteins, and ancillary reagents for chemokine and platelet-related targets. Whether you need consistent lot-to-lot antibody supplies, custom formulations, or assistance in identifying rare reagents, CamelBio is equipped to support your project from concept to clinic.