Anti-PPARD Rabbit Polyclonal Antibody for WB and ELISA - Q03181

Core Product Specifications and Parameters

Biological Background: PPARD Function and Localization

• PPARD (Peroxisome proliferator-activated receptor delta) is also known as PPAR-beta, NUCI, NUC1, NR1C2, NUCII, and FAAR.
• It functions as a ligand-activated transcription factor and is a key mediator of energy metabolism in adipose tissues. Related references: PMID:35675826
• PPARD binds peroxisome proliferators such as hypolipidemic drugs and fatty acids, with preference for polyunsaturated fatty acids like gamma-linoleic acid and eicosapentaenoic acid.
• Upon ligand activation, PPARD binds to promoter elements of target genes, regulating the peroxisomal beta-oxidation pathway of fatty acids and activating acyl-CoA oxidase gene expression.
• It decreases expression of NPC1L1 once activated by a ligand, potentially impacting cholesterol absorption.
• PPARD is localized to the nucleus, consistent with its role as a nuclear receptor transcription factor.
• It is ubiquitously expressed, with highest levels in placenta and skeletal muscle.
• The protein is subject to alternative splicing, DNA-binding, zinc-binding, and Ubl conjugation, as indicated by UniProt keywords.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide from amino acids 101-200 of human PPARD. For Western blot, consider using the predicted molecular weight of approximately 50 kDa as a reference. Validate with positive and negative controls in relevant tissue lysates.
• For ELISA, optimize coating antigen concentration and antibody dilution; consider using recombinant PPARD protein or peptide.
• Cross-reactivity with rat PPARD has been observed; validate in rat samples if applicable to your research.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw-material procurement by providing a comprehensive portfolio of validated antibodies, including this anti-PPARD polyclonal antibody for metabolic and nuclear receptor research. Beyond single products, we support diagnostic manufacturers with bulk ancillary reagents, optimized antibody pairs, and sourcing for rare or custom targets, helping accelerate your assay development from concept to clinic.