Anti-PPARA Rabbit Polyclonal Antibody for WB - Q07869

Core Product Specifications and Parameters

Biological Background: PPARA Function and Localization

• PPARA is a ligand-activated transcription factor belonging to the nuclear receptor subfamily 1 group C member 1, also known as PPARalpha, NR1C1, or hPPAR.
• It is a key regulator of lipid metabolism, activated by endogenous ligands such as 1-palmitoyl-2-oleoyl-sn-glycerol-3-phosphocholine (16:0/18:1-GPC) and oleoylethanolamide, a lipid that regulates satiety.
• Functions as a receptor for peroxisome proliferators, including hypolipidemic drugs and fatty acids, and regulates the peroxisomal beta-oxidation pathway of fatty acids.
• Transactivation activity requires heterodimerization with the retinoid X receptor alpha (RXRA) and is antagonized by NR2C2.
• PPARA acts as a transcription activator for genes such as ACOX1 and P450, and may help propagate circadian clock information to metabolic pathways via PER2.
• Predominantly localized in the nucleus, with tissue-specific expression in skeletal muscle, liver, heart, kidney, and monocytes.
• Contains conserved zinc-finger DNA-binding domains, metal-binding sites, and is subject to alternative splicing and ubiquitin-like conjugation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a conserved region in human PPARA; cross-reactivity with mouse is reported and should be validated in your experimental system.
• For Western blot, consider enriching nuclear fractions to enhance detection and include appropriate controls to account for polyclonal antibody variability.
• The predicted protein weight is 52 kDa; however, post-translational modifications or alternative splicing may result in additional bands.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, offering validated antibody pairs, optimized polyclonal antibodies, and bulk ancillary reagents. This anti-PPARA antibody supports research in lipid metabolism and nuclear receptor pathways, and CamelBio can assist with sourcing custom antibodies, recombinant proteins, and other related reagents tailored to your diagnostic development needs.